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4dn:phase1:2017_annual_meeting:integrated_approaches_workshop
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| - | <font 13.3333px/arial;;initial;;#ffffff>**WORKSHOP**</font> | ||
| - | <font 13.3333px/arial;;initial;;#ffffff>**Integrated Approaches for Spatial and Temporal Mapping of Gene Activity in Cells and Tissues**</font> | ||
| - | <font 13.3333px/arial;;initial;;#ffffff>SEPTEMBER 20, 2017</font> | + | **WORKSHOP** |
| - | <font 13.3333px/arial;;initial;;#ffffff>LOCATION: Bethesda North Marriott Hotel ( CKGE_TMP_i Salon F-G CKGE_TMP_i )</font> | + | **Integrated Approaches for Spatial and Temporal Mapping of Gene Activity in Cells and Tissues** |
| - | [[https://docs.google.com/document/d/1yOrIYJYOVdIHwdttpT5mx2523z9AiyXgdHWiXG06LJM/edit?usp=sharing|PROGRAM AGENDA]] | + | SEPTEMBER 20, 2017 |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | LOCATION: Bethesda North Marriott Hotel (Salon F-G) |
| - | <font 13.3333px/arial;;initial;;#ffffff>1:00 Introductory statements; Rob Singer</font> | + | [[https://docs.google.com/document/d/1yOrIYJYOVdIHwdttpT5mx2523z9AiyXgdHWiXG06LJM/edit?usp=sharing|PROGRAM AGENDA]] |
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| - | <font 13.3333px/arial;;initial;;#ffffff>The purpose of this workshop is to introduce expertise and technology to the consortium that would be important to help achieve the 4DN goals. The workshop will focus on spatial and temporal aspects of chromatin structure that lead to activation. **The goal would be to: a) identify areas of collaboration for the 4DN network, 2) identify potential areas of collaboration with other programs, 3) provide an opportunity to have a dialog between 4DN investigators and other researchers working in this field.**</font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff>1:05 Gary Karpen (Berkeley). **"Phase transitions drive chromatin domain formation"**</font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff>Gary has been assessing the role of chromatin in gene activation. His recent work demonstrates that chromatin domains undergo phase transitions. ( CKGE_TMP_i Nature CKGE_TMP_i 2017, PMID:28636597)</font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff>1:30 Xiaowei Zhuang (Harvard**). “Imaging the spatial organization of the chromosome and the transcriptome in single cells”.**</font> | + | |
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| - | <font 13.3333px/arial;;initial;;#ffffff>Xiaowei has been developing superresolution methods for high resolution imaging of chromatin and their compartments over time ( CKGE_TMP_i Science CKGE_TMP_i 2016, PMCID PMC4991974)</font> | + | |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | 1:00 Introductory statements; Rob Singer |
| - | <font 13.3333px/arial;;initial;;#ffffff>2:00 Michael Levine (Princeton) **“Promoter-enhancer interactions as a model for gene activation”**</font> | + | * The purpose of this workshop is to introduce expertise and technology to the consortium that would be important to help achieve the 4DN goals. The workshop will focus on spatial and temporal aspects of chromatin structure that lead to activation. The goal would be to: a) identify areas of collaboration for the 4DN network, 2) identify potential areas of collaboration with other programs, 3) provide an opportunity to have a dialog between 4DN investigators and other researchers working in this field. |
| - | <font 13.3333px/arial;;initial;;#ffffff>Michael has been investigating the spatial and temporal events concerning promoters and enhancers in living embryos. They stand as a model for how 4D DNA folding can activate transcriptional bursting ( CKGE_TMP_i Cell CKGE_TMP_i 2016, PMCID:PMC4970759)</font> | + | 1:05 Gary Karpen (Berkeley). **"Phase transitions drive chromatin domain formation"** |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | * Gary has been assessing the role of chromatin in gene activation. His recent work demonstrates that chromatin domains undergo phase transitions. (//Nature// 2017, PMID:28636597) |
| - | <font 13.3333px/arial;;initial;;#ffffff>2:30 break</font> | + | 1:30 Xiaowei Zhuang (Harvard). **“Imaging the spatial organization of the chromosome and the transcriptome in single cells”.** |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | * Xiaowei has been developing superresolution methods for high resolution imaging of chromatin and their compartments over time (//Science //2016, PMCID PMC4991974) |
| - | <font 13.3333px/arial;;initial;;#ffffff>3:00 Rifka Vlijm (Heidelberg) **“Imaging nuclear structural changes using STED microscopy”.**</font> | + | 2:00 Michael Levine (Princeton) **“Promoter-enhancer interactions as a model for gene activation”** |
| - | <font 13.3333px/arial;;initial;;#ffffff>Rifka has been working with Stefan Hell to develop dyes for superresolution STED microscopy for investigating nuclear events in living cells and embryos. ( CKGE_TMP_i Chemistry CKGE_TMP_i , 2017, PMID 28370443)</font> | + | * Michael has been investigating the spatial and temporal events concerning promoters and enhancers in living embryos. They stand as a model for how 4D DNA folding can activate transcriptional bursting (//Cell// 2016, PMCID:PMC4970759) |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | 2:30 break |
| - | <font 13.3333px/arial;;initial;;#ffffff>3:30 Luke Lavis (Janelia Research Campus) **"New dyes for imaging nuclear structures in real time".**</font> | + | 3:00 Rifka Vlijm (Heidelberg) ** “Imaging nuclear structural changes using STED microscopy”.** |
| - | <font 13.3333px/arial;;initial;;#ffffff>Luke is part of the senior leadership at Janelia. He is a leading organic chemist and responsible for generating the fluorogenic JF dyes that are now widely used for biological imaging. He is currently developing new variants that are brighter with lower phototoxicity. These dyes will eventually make it possible to image cells for longer times with less photodamage ( CKGE_TMP_i Nature Methods CKGE_TMP_i 2016, PMID:27776112)</font> | + | * Rifka has been working with Stefan Hell to develop dyes for superresolution STED microscopy for investigating nuclear events in living cells and embryos. (//Chemistry//, 2017, PMID 28370443) |
| - | <font 13.3333px/arial;;initial;;#ffffff></font> | + | 3:30 Luke Lavis (Janelia Research Campus)** "New dyes for imaging nuclear structures in real time".** |
| - | <font 13.3333px/arial;;initial;;#ffffff>4:00 Discussion: Joan Ritland, Job Dekker, Andy Belmont, David Gilbert, Jan Liphardt, Rob Singer and Richard Conroy</font> | + | * Luke is part of the senior leadership at Janelia. He is a leading organic chemist and responsible for generating the fluorogenic JF dyes that are now widely used for biological imaging. He is currently developing new variants that are brighter with lower phototoxicity. These dyes will eventually make it possible to image cells for longer times with less photodamage (//Nature Methods// 2016, PMID:27776112) |
| - | <font 13.3333px/arial;;initial;;#ffffff>** The purpose of this session would be to discuss and come up with suggested Consortium activities for: 1) the temporal dynamics of nuclear architecture, 2) nuclear structure in primary cells in a 3D tissue environment.</font>** | + | 4:00 Discussion: Joan Ritland, Job Dekker, Andy Belmont, David Gilbert, Jan Liphardt, Rob Singer and Richard Conroy |
| - | <font 13.3333px/arial;;initial;;#ffffff>5:00 Adjourn</font> | + | * ** The purpose of this session would be to discuss and come up with suggested Consortium activities for: 1) the temporal dynamics of nuclear architecture, 2) nuclear structure in primary cells in a 3D tissue environment. ** |
| + | 5:00 Adjourn | ||
4dn/phase1/2017_annual_meeting/integrated_approaches_workshop.1548271053.txt.gz · Last modified: 2025/04/22 16:21 (external edit)