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4dn:phase1:omicswg:minutes-03-13-2017
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4dn:phase1:omicswg:minutes-03-13-2017 [2019/02/15 10:19] oh created |
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| - | ==== Omics Data Standards WG - Minutes 03-13-2017 ==== | ||
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| - | |~~TABLE_CELL_WRAP_START~~<WRAP> | ||
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| ==== Omics Data Standards WG - Minutes 03-13-2017 ==== | ==== Omics Data Standards WG - Minutes 03-13-2017 ==== | ||
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| Boundary resolution | Boundary resolution | ||
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| 4DN Goal: define what resolution means | 4DN Goal: define what resolution means | ||
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| 2.- E/L method | 2.- E/L method | ||
| Different cell lines have different data qualities and resolutions as well | Different cell lines have different data qualities and resolutions as well | ||
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| DNA labeling: BrdU vs EdU. PFA Fixation affects the map | DNA labeling: BrdU vs EdU. PFA Fixation affects the map | ||
| Quality controls list | Quality controls list | ||
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| Variation in sized in a typical experiment (for QC) | Variation in sized in a typical experiment (for QC) | ||
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| 2.- Processing E/L method | 2.- Processing E/L method | ||
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| Shape recognition for “ripples”, are those discontinuities in replication domains? | Shape recognition for “ripples”, are those discontinuities in replication domains? | ||
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| To what extent the resolution is limited by cell-to-cell variation instead of technology. Combination of single cells experiments are needed | To what extent the resolution is limited by cell-to-cell variation instead of technology. Combination of single cells experiments are needed | ||
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| 3. Repli-chip vs Repli-seq | 3. Repli-chip vs Repli-seq | ||
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| In most of the cases Repli-chip is adequate but not all cases. | In most of the cases Repli-chip is adequate but not all cases. | ||
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| 4. Repli-seq biases and QC | 4. Repli-seq biases and QC | ||
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| Are there additional information that will be useful to evaluate Repli-seq data? Those data can be included when depositing Repli-seq data. A second session can be scheduled to discuss about these. | Are there additional information that will be useful to evaluate Repli-seq data? Those data can be included when depositing Repli-seq data. A second session can be scheduled to discuss about these. | ||
| - | </WRAP>~~TABLE_CELL_WRAP_STOP~~| | ||
| </WRAP>~~TABLE_CELL_WRAP_STOP~~| | </WRAP>~~TABLE_CELL_WRAP_STOP~~| | ||
4dn/phase1/omicswg/minutes-03-13-2017.1550254760.txt.gz · Last modified: 2025/04/22 16:21 (external edit)