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4dn:phase1:protocols [2019/01/24 15:50]
rcalandrelli created
4dn:phase1:protocols [2025/04/22 16:21] (current)
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-a+====== Protocols ====== 
 + 
 +This page serves as repository of protocols used in 4DN projects. All teams are encouraged to post your protocols as sub-pages to this Wiki page. 
 + 
 +1. [[https://​docs.google.com/​document/​d/​1N8ZgdQen2VFO30vyk4fHlpKko18bZIaYfQdbtordhnU/​edit|Genomic technologies currently in use or being developed in the 4DN network]] (10/​17/​2016). 
 + 
 +  - 3C-seq ([[http://​download.springer.com/​static/​pdf/​909/​chp:​10.1007/​978-1-60327-461-6_7.pdf?​originUrl=http://​link.springer.com/​protocol/​10.1007/​978-1-60327-461-6_7&​token2=exp=1478559619~acl=/​static/​pdf/​909/​chp%3A10.1007%2F978-1-60327-461-6_7.pdf?​originUrl=http://​link.springer.com/​protocol/​10.1007/​978-1-60327-461-6_7*~hmac=eb2a0496e877ac93bc53ff29375c9a27fe2ada021c8f91470c0a85d883ae10f0|Dekker lab]]) 
 + 
 +  - 4C-seq ([[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyZTQ0ZDU1NDE0YTRjZGMy|Harmen J.G. van Werken et al. 2012]]) 
 + 
 +  - 5C ([[http://​www.nature.com/​nprot/​journal/​v2/​n4/​pdf/​nprot.2007.116.pdf|J. Dostie & J. Dekker]][[http://​www.nature.com/​nprot/​journal/​v2/​n4/​pdf/​nprot.2007.116.pdf|http://​www.nature.com/​nprot/​journal/​v2/​n4/​pdf/​nprot.2007.116.pdf]]. 2007) 
 + 
 +  - Hi-C ([[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDozOTVjOGZkYjNlZmJhNTdj|Dekker. lab, supp data]]) 
 + 
 +  - ChIA-PET ([[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyZWY2OWFkY2Y4ZWZkMQ|Y. Goh & Ruan et al 2012]]) 
 + 
 +  - Capture-Hi-C ([[http://​www.nature.com/​articles/​ncomms7178#​methods|Houlston lab, 2015]]) 
 + 
 +  - Tethered Conformation Capture (TCC)- ([[http://​www.nature.com/​nbt/​journal/​v30/​n1/​extref/​nbt.2057-S1.pdf|R. Kalhor & L. Chen et al Nat 2012]], supp data p38) 
 + 
 +  - In situ Hi-C ([[http://​www.cell.com/​cms/​attachment/​2033493464/​2049506921/​mmc1.pdf|Rao and Erez-Lieberman A. 2014]], methods p18) 
 + 
 +  - Dnase Hi-C ([[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo1M2ZkZTRhODJkNjg5N2Zm|Ramani et al 2016)]] 
 + 
 +  - Micro-C ([[http://​www.nature.com/​protocolexchange/​protocols/​5105#/​procedure|Rando Lab, Procedure]]) 
 + 
 +  - Genome Architecture Mapping (GAM) (2017 [[https://​www.nature.com/​articles/​nature21411|research paper]]) 
 + 
 +  - COLA ([[https://​www.ncbi.nlm.nih.gov/​pmc/​articles/​PMC4064277/​pdf/​1751-0473-9-12.pdf|Grabherr lab, 2014]] (full paper), [[http://​scfbm.biomedcentral.com/​articles/​10.1186/​1751-0473-9-12#​Sec2|methods]] 
 + 
 +  - Single-cell Hi-C ([[http://​www.nature.com/​nprot/​journal/​v10/​n12/​pdf/​nprot.2015.127.pdf|Frazer Lab, 2013]]) 
 + 
 +  - Combinatorial single-cell Hi-C ([[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyMGFmMDNjNTQ1Zjg2MTgz|Shendure Lab, 2015]] (paper), [[http://​science.sciencemag.org/​content/​sci/​suppl/​2015/​05/​06/​science.aab1601.DC1/​Cusanovich-SM.pdf|methods ]](supp data)) 
 + 
 +  - Split-pool Barcoding ([[http://​www.cell.com/​cms/​attachment/​2032378908/​2048933656/​mmc7.pdf|McCarroll Lab, 2015]]) 
 + 
 +  - RICh-PET(Ruan Lab, Unpublished) 
 + 
 +  - ChIP RICh-PET (Ruan Lab, Unpublished) 
 + 
 +  - Distance Hi-C (Zipfeld Lab, Unpublished) 
 + 
 +  - [[https://​sites.google.com/​a/​4dnucleome.org/​4d-nucleome-wiki/​protocols/​damid|DamID]] (Bas van Steensel, 2000 ([[http://​www.nature.com/​nbt/​journal/​v18/​n4/​pdf/​nbt0400_424.pdf|research paper]]), 2007 ([[http://​www.nature.com/​nprot/​journal/​v2/​n6/​pdf/​nprot.2007.148.pdf|Protocol]]) and Single-Cell DamID (van Steensel Lab, 2013 ([[http://​www.sciencedirect.com/​science/​article/​pii/​S0092867413002171|extended experimental pro]]cedures) 
 + 
 +  - TSA-Seq ([[https://​www.ncbi.nlm.nih.gov/​genbank/​tsa/​|U]]npublished) 
 + 
 +  - Repli-Seq ([[http://​www.biorxiv.org/​content/​early/​2017/​03/​01/​104653|Marchal et. al., Nature Protocols, in press 2017]]) 
 + 
 +  - TRIP ([[http://​www.nature.com/​nprot/​journal/​v9/​n6/​pdf/​nprot.2014.072.pdf|Bas van Steensel & Maarten van Lohuizen, 2014]], Protocol) 
 + 
 +  - [[https://​docs.google.com/​a/​eng.ucsd.edu/​viewer?​a=v&​pid=sites&​srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo1OGFkMTRkYmY5ZjI0NzQw|CUT&​RUN (Steven Henikoff 2017)]] 
 + 
 +2. Imaging technologies currently in use or being developed in the 4DN network (being compiled by Clodagh O'​Shea). 
 + 
 +-------------------------------------- 
 + 
 +{{medialist>​4dn:​protocols:​}}
  
  
4dn/phase1/protocols.1548373819.txt.gz · Last modified: 2025/04/22 16:21 (external edit)