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4dn:phase1:working_groups:imaging_data_standards:microscope_calibration_project
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4dn:phase1:working_groups:imaging_data_standards:microscope_calibration_project [2019/02/14 11:35] rcalandrelli created |
4dn:phase1:working_groups:imaging_data_standards:microscope_calibration_project [2025/04/22 16:21] (current) |
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| ====== Microscope Calibration Project ====== | ====== Microscope Calibration Project ====== | ||
| - | {{:4dn:microscope.png?nolink&1114x371}}\\ | + | {{4dn:phase1:microscope.png?nolink&800x266}} |
| ===== Introduction ===== | ===== Introduction ===== | ||
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| This is the current (October 2017 to ~March 2018) project part. The aim is to establish a database with monthly recorded multi-color bead stacks from microscopes within the 4DN consortium. Bead stacks are used for two reasons: | This is the current (October 2017 to ~March 2018) project part. The aim is to establish a database with monthly recorded multi-color bead stacks from microscopes within the 4DN consortium. Bead stacks are used for two reasons: | ||
| - | - | + | - To create a reference data set that can be used to compare results from the microscope of any specific user against other comparable microscopes. |
| - | To create a reference data set that can be used to compare results from the microscope of any specific user against other comparable microscopes. | + | - As an easy sample with minimal variability to work with DCIC on metadata organization and data upload. |
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| - | - | + | |
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| - | As an easy sample with minimal variability to work with DCIC on metadata organization and data upload. | + | |
| Over the duration of this project we will expand the number of contributors. We start with the Grunwald lab and DCIC (aim: develop the technology and infrastructure backbone), step one expansion will include a small set of optical technology development labs (aim: beta test the system), in step two we reach out to image production labs (aim: calling the afford) and in step three will address everyone using a microscope in the consortium. | Over the duration of this project we will expand the number of contributors. We start with the Grunwald lab and DCIC (aim: develop the technology and infrastructure backbone), step one expansion will include a small set of optical technology development labs (aim: beta test the system), in step two we reach out to image production labs (aim: calling the afford) and in step three will address everyone using a microscope in the consortium. | ||
| - | **IF YOU WANT TO CONTRIBUTE TODAY:** Everybody is welcome and invited to join this project at anytime. Please download the one-page protocol below (under "Optical Calibration Documents") to get started. Contact me (david.grunwald@umassmed.edu) with questions and start taking bead stacks! The above steps are a lower bound: we will bother you to contribute at some point, we welcome you at any time! | + | **IF YOU WANT TO CONTRIBUTE TODAY:** Everybody is welcome and invited to join this project at anytime. Please download the one-page protocol below (under "Optical Calibration Documents") to get started. Contact me (david.grunwald@umassmed.edu) with questions and start taking bead stacks! The above steps are a lower bound: we will bother you to contribute at some point, we welcome you at any time! |
| ===== Optical Calibration - Definition and Resources ===== | ===== Optical Calibration - Definition and Resources ===== | ||
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| ==== Optical Calibration Documents ==== | ==== Optical Calibration Documents ==== | ||
| - | * | + | * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/imaging-data-standards-wg/microscope-calibration-project/literature|LITERATURE]] |
| - | [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/imaging-data-standards-wg/microscope-calibration-project/literature|LITERATURE]] | + | * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/imaging-data-standards-wg/microscope-calibration-project/optical-calibration-documents|Optical Calibration Documents]] |
| - | * | + | ------------------------------------------------------------------------------------------ |
| - | [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/imaging-data-standards-wg/microscope-calibration-project/optical-calibration-documents|Optical Calibration Documents]] | + | {{medialist>4dn:working_groups:imaging_data_standards:microscope_calibration_project:}} |
4dn/phase1/working_groups/imaging_data_standards/microscope_calibration_project.1550172905.txt.gz · Last modified: 2025/04/22 16:21 (external edit)