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4dn:phase2:4dn_cell_repository1
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4dn:phase2:4dn_cell_repository1 [2020/12/14 09:05] rcalandrelli created |
4dn:phase2:4dn_cell_repository1 [2025/04/22 16:21] (current) |
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| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
| - | **Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC** | + | <font inherit/inherit;;#3498db;;inherit>**Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC**</font> |
| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
| - | __**Eligibility information for PIs who are new to 4DN Cell Repository~~CKG_TABLE_NBSP~~**__ | + | <font inherit/inherit;;#c0392b;;inherit>__**Eligibility information for PIs who are new to 4DN Cell Repository~~CKG_TABLE_NBSP~~**__</font> |
| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
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| [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | ||
| - | **ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?**- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]]~~CKG_TABLE_NBSP~~of who is engineering what so as not to duplicate effort or work together! | + | <font inherit/inherit;;#c0392b;;inherit>**ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?**</font>- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]]~~CKG_TABLE_NBSP~~of who is engineering what so as not to duplicate effort or work together! |
| ===== How to Order Tier 1 and Tier 2 Cells ===== | ===== How to Order Tier 1 and Tier 2 Cells ===== | ||
| - | **IMPORTANT!**~~CKG_TABLE_NBSP~~The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. | + | <font inherit/inherit;;#c0392b;;inherit>**IMPORTANT!**</font>~~CKG_TABLE_NBSP~~The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. |
| **Before thawing the cells**\\ \\ **Read the finalized SOP for your cell line from** ~~CKG_TABLE_NBSP~~[[https://wiki.4dnucleome.org/4dn:working_groups:cell_lines_and_samples|Cell lines WG wiki page]].~~CKG_TABLE_NBSP~~The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. | **Before thawing the cells**\\ \\ **Read the finalized SOP for your cell line from** ~~CKG_TABLE_NBSP~~[[https://wiki.4dnucleome.org/4dn:working_groups:cell_lines_and_samples|Cell lines WG wiki page]].~~CKG_TABLE_NBSP~~The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. | ||
| - | **DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!** | + | <font inherit/inherit;;#c0392b;;inherit>**DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!**</font> |
| - | \\ **1.~~CKG_TABLE_NBSP~~Human embryonic stem cells H1**, by WiCell.~~CKG_TABLE_NBSP~~AVAILABLE | + | \\ **1.~~CKG_TABLE_NBSP~~Human embryonic stem cells H1**, by WiCell.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN%20WiCell%20Ordering%20Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]]~~CKG_TABLE_NBSP~~([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]])~~CKG_TABLE_NBSP~~August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost. | * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN%20WiCell%20Ordering%20Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]]~~CKG_TABLE_NBSP~~([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]])~~CKG_TABLE_NBSP~~August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost. | ||
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| * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]] | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]] | ||
| - | Request a second vial: | + | <font inherit/inherit;;#c0392b;;inherit>Request a second vial:</font> |
| ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Tennielle Ludwig from WiCell ([[tludwig@wicell.org|]]). After you have send the letter the order procedure is the same. | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Tennielle Ludwig from WiCell ([[tludwig@wicell.org|]]). After you have send the letter the order procedure is the same. | ||
| - | **2.~~CKG_TABLE_NBSP~~GM12878**, reserved lot by Coriell:~~CKG_TABLE_NBSP~~AVAILABLE | + | **2.~~CKG_TABLE_NBSP~~GM12878**, reserved lot by Coriell:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase | ~~CKG_TABLE_NBSP~~Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase | ||
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| * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]09, 2018 | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]09, 2018 | ||
| - | ~~CKG_TABLE_NBSP~~Request a second vial: | + | <font inherit/inherit;;#c0392b;;inherit>~~CKG_TABLE_NBSP~~Request a second vial:</font> |
| ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]])~~CKG_TABLE_NBSP~~and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]).~~CKG_TABLE_NBSP~~After you have send the letter the order procedure is the same. | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]])~~CKG_TABLE_NBSP~~and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]).~~CKG_TABLE_NBSP~~After you have send the letter the order procedure is the same. | ||
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| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
| - | **3.~~CKG_TABLE_NBSP~~IMR90**, reserved lot by ATCC:~~CKG_TABLE_NBSP~~AVAILABLE | + | **3.~~CKG_TABLE_NBSP~~IMR90**, reserved lot by ATCC:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]] | * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]] | ||
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| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]] | ||
| - | Process for international customers: | + | <font inherit/inherit;;#c0392b;;inherit>Process for international customers:</font> |
| ~~CKG_TABLE_NBSP~~__ Step 1:__ | ~~CKG_TABLE_NBSP~~__ Step 1:__ | ||
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| ~~CKG_TABLE_NBSP~~3. ATCC international order entry team will process the order. | ~~CKG_TABLE_NBSP~~3. ATCC international order entry team will process the order. | ||
| - | ~~CKG_TABLE_NBSP~~Request a second vial: | + | <font inherit/inherit;;#c0392b;;inherit>~~CKG_TABLE_NBSP~~Request a second vial:</font> |
| ~~CKG_TABLE_NBSP~~Request a letter of consent from Dr. David Gilbert ([[gilbert@bio.fsu.edu|]])~~CKG_TABLE_NBSP~~and send it by email to Stefan Brings from ATCC ([[sbrings@atcc.org|]]) | ~~CKG_TABLE_NBSP~~Request a letter of consent from Dr. David Gilbert ([[gilbert@bio.fsu.edu|]])~~CKG_TABLE_NBSP~~and send it by email to Stefan Brings from ATCC ([[sbrings@atcc.org|]]) | ||
| - | **4.~~CKG_TABLE_NBSP~~HFF-hTert-clone 6 reserved lot from UMass**.~~CKG_TABLE_NBSP~~AVAILABLE | + | **4.~~CKG_TABLE_NBSP~~HFF-hTert-clone 6 reserved lot from UMass**.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. | ~~CKG_TABLE_NBSP~~The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. | ||
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| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]] | ||
| - | **5.~~CKG_TABLE_NBSP~~F121-9 hybrid CASTx129 Female Mouse ESC**: AVAILABLE | + | **5.~~CKG_TABLE_NBSP~~F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.~~CKG_TABLE_NBSP~~ | Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.~~CKG_TABLE_NBSP~~ | ||
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| ~~CKG_TABLE_NBSP~~**NOTE**: ShojiTakahashi~~CKG_TABLE_NBSP~~is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | ~~CKG_TABLE_NBSP~~**NOTE**: ShojiTakahashi~~CKG_TABLE_NBSP~~is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | ||
| - | **6.~~CKG_TABLE_NBSP~~F123 hybrid CASTx129 Male Mouse ESC**: AVAILABLE | + | **6.~~CKG_TABLE_NBSP~~F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| Contact Bing Ren for vials or with questions.~~CKG_TABLE_NBSP~~ | Contact Bing Ren for vials or with questions.~~CKG_TABLE_NBSP~~ | ||
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| ~~CKG_TABLE_NBSP~~NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | ~~CKG_TABLE_NBSP~~NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | ||
| - | **7. HAP1 Cells:**~~CKG_TABLE_NBSP~~AVAILABLE | + | **7. HAP1 Cells:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. | ~~CKG_TABLE_NBSP~~HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. | ||
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| ~~CKG_TABLE_NBSP~~The PI is responsible of the shipping cost | ~~CKG_TABLE_NBSP~~The PI is responsible of the shipping cost | ||
| - | ~~CKG_TABLE_NBSP~~To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl ]] | + | ~~CKG_TABLE_NBSP~~To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl ]] |
| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] | ||
| - | **8.~~CKG_TABLE_NBSP~~Human embryonic stem cells H9 (female)**, by WiCell.~~CKG_TABLE_NBSP~~AVAILABLE | + | **8.~~CKG_TABLE_NBSP~~Human embryonic stem cells H9 (female)**, by WiCell.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from WiCell, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from WiCell, use SOP listed on Cells Wiki | ||
| - | **9. hTERT-RPE:**~~CKG_TABLE_NBSP~~AVAILABLE | + | **9. hTERT-RPE:** <font inherit/inherit;;#c0392b;;inherit>~~CKG_TABLE_NBSP~~AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ||
| - | **10. K562:~~CKG_TABLE_NBSP~~** AVAILABLE | + | **10. K562:~~CKG_TABLE_NBSP~~** <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ||
| - | **11. HEK293:**~~CKG_TABLE_NBSP~~AVAILABLE | + | **11. HEK293:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ||
| - | **12.~~CKG_TABLE_NBSP~~WTC-11**:~~CKG_TABLE_NBSP~~AVAILABLE | + | **12.~~CKG_TABLE_NBSP~~WTC-11**:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from Coriell, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from Coriell, use SOP listed on Cells Wiki | ||
| - | **13. U2OS:**~~CKG_TABLE_NBSP~~AVAILABLE | + | **13. U2OS:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki | ||
| - | **14. JM8.N4**: AVAILABLE | + | **14. JM8.N4**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Obtain from KOMP, use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Obtain from KOMP, use SOP listed on Cells Wiki | ||
| - | **15. G4 mESC:**~~CKG_TABLE_NBSP~~AVAILABLE | + | **15. G4 mESC:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font> |
| ~~CKG_TABLE_NBSP~~Contact Carol Ware from the ISCRM-University of Washinton for G4 mESC distribution [[cware@u.washington.edu|]]~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~Contact Carol Ware from the ISCRM-University of Washinton for G4 mESC distribution [[cware@u.washington.edu|]]~~CKG_TABLE_NBSP~~ | ||
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| ~~CKG_TABLE_NBSP~~Use SOP listed on Cells Wiki | ~~CKG_TABLE_NBSP~~Use SOP listed on Cells Wiki | ||
| - | |||
| </WRAP>~~TABLE_CELL_WRAP_STOP~~| | </WRAP>~~TABLE_CELL_WRAP_STOP~~| | ||
| |~~CKG_TABLE_NBSP~~| | |~~CKG_TABLE_NBSP~~| | ||
4dn/phase2/4dn_cell_repository1.1607965550.txt.gz · Last modified: 2025/04/22 16:21 (external edit)