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4dn:phase2:4dn_cell_repository_4
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4dn:phase2:4dn_cell_repository_4 [2021/03/09 11:02] oh [4DN Cell Repository] |
4dn:phase2:4dn_cell_repository_4 [2025/04/22 16:21] (current) |
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| <font 16px/inherit;;inherit;;inherit>Please note that there are additional cell lines that are under discussion by the Cell Line WG.</font> | <font 16px/inherit;;inherit;;inherit>Please note that there are additional cell lines that are under discussion by the Cell Line WG.</font> | ||
| - | <font inherit/inherit;;#2980b9;;inherit>**<font 18px/inherit;;inherit;;inherit>Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC</font>** </font> | + | <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#2980b9;;inherit>**Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC**</font></font> |
| + | <font 16px/inherit;;inherit;;inherit>**<font inherit/inherit;;#c0392b;;inherit>__Eligibility information for PIs who are new to 4DN Cell Repository__</font>** </font> | ||
| - | ===== <font inherit/inherit;;#c0392b;;inherit>Eligibility information for PIs who are new to 4DN Cell Repository</font> ===== | + | All current Phase II PIs have been approved by the Phase II 4DN Cells WG to receive a single vial of each of our commercial repository stocked Tier1 cell lines, including: H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC). Additional vials will require separate approval from the Cells WG. |
| - | <font 16px/inherit;;inherit;;inherit>Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <[[gilbert@bio.fsu.edu|]]>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <[[oh@4ducleome.org|oh@4dnucleome.org]]>, a member of the 4DN Cell Line Working group.</font> | + | **NOTE: **All PIs are reminded to stock the cells by 4DN standards and to follow the 4DN approved SOP for all cell lines. When data will be submitted to the DCIC, they will need to verify that the cells have been expanded in these conditions that are posted on the Cells WG Wiki page, and the appropriate metadata will need to be recorded at the time of cell harvest in order for datasets to receive the gold standard flag. |
| - | <font 16px/inherit;;inherit;;inherit>**Content of your request letter**</font> | + | **[[https://drive.google.com/file/d/1Iq0dWUl6zLIQkYcAMFWMoaGFSPOLFxVK/view?usp=sharing|Copy of the consent letter for Phase II PIs]]** |
| - | + | ||
| - | <font 16px/inherit;;inherit;;inherit>In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. **Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.**</font> | + | |
| - | + | ||
| - | <font 16px/inherit;;inherit;;inherit>**Review of your letter**</font> | + | |
| - | + | ||
| - | <font 16px/inherit;;inherit;;inherit>After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the “current PI list who have requested this cell line” on 4DN wiki.</font> | + | |
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| - | ===== ===== | + | |
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| [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | ||
| - | **ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?**- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]] of who is engineering what so as not to duplicate effort or work together! | ||
| ===== How to Order Tier 1 and Tier 2 Cells ===== | ===== How to Order Tier 1 and Tier 2 Cells ===== | ||
| - | **IMPORTANT!** The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. | + | <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>**IMPORTANT!**</font>The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells.</font> |
| - | + | ||
| - | **Before thawing the cells**\\ \\ **Read the finalized SOP for your cell line from** [[https://wiki.4dnucleome.org/4dn:phase2:working_groups:cell_lines_and_samples|Cell lines WG wiki page]]. The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. | + | |
| - | + | ||
| - | **DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED “GOLD STANDARD” - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED “GOLD STANDARD”. MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!** | + | |
| - | + | ||
| - | \\ **1. Human embryonic stem cells H1**, by WiCell. AVAILABLE | + | |
| - | + | ||
| - | * | + | |
| - | + | ||
| - | [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN%20WiCell%20Ordering%20Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]] ([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]]) August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost. | + | |
| - | + | ||
| - | * | + | |
| - | + | ||
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]] | + | |
| - | + | ||
| - | Request a second vial: | + | |
| - | + | ||
| - | Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Tennielle Ludwig from WiCell ([[tludwig@wicell.org|]]). After you have send the letter the order procedure is the same. | + | |
| - | + | ||
| - | **2. GM12878**, reserved lot by Coriell: AVAILABLE | + | |
| - | + | ||
| - | Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase | + | |
| - | + | ||
| - | * | + | |
| - | + | ||
| - | [[https://docs.google.com/document/d/1wFZh5LbuMlXCAWMHKwk0aH0d7ZqLmaNnwDzh0mip_ko/edit?usp=sharing|Coriell Ordering Instructions (GM12878)]] | + | |
| - | + | ||
| - | * | + | |
| - | + | ||
| - | [[https://docs.google.com/document/d/1kwYAUGBsuqs0JQ-TrrJ-pJ5MtyDWqoP9AwT-lw21ZVw/edit|Protocol to Order GM12878 Cells from Coriell]] (slides) | + | |
| - | + | ||
| - | * | + | |
| - | + | ||
| - | //International Orders:// The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is customerservice@coriell.org[[customerservice@coriell.org|]] or call 1-856-757-4848. | + | |
| - | + | ||
| - | * | + | |
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]09, 2018 | + | <font 16px/inherit;;inherit;;inherit>**Before thawing the cells**</font>\\ \\ <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>**Read the finalized SOP for your cell line from**</font></font> [[https://wiki.4dnucleome.org/4dn:phase2:working_groups:cell_lines_and_samples|Cell lines WG wiki page]]. The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. |
| - | Request a second vial: | + | <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>**DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED “GOLD STANDARD” - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED “GOLD STANDARD”. MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!**</font></font> |
| - | Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]). After you have send the letter the order procedure is the same. | + | \\ <font 16px/inherit;;inherit;;inherit>**1. Human embryonic stem cells H1**, by WiCell. <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | **3. IMR90**, reserved lot by ATCC: AVAILABLE | + | * <font 16px/inherit;;inherit;;inherit>[[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN%20WiCell%20Ordering%20Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]] ([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]]) August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost</font> |
| + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]]</font> | ||
| - | * | + | <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>Request a second vial:</font></font> |
| - | [[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]] | + | <font 16px/inherit;;inherit;;inherit>Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Tennielle Ludwig from WiCell ([[tludwig@wicell.org|]]). After you have send the letter the order procedure is the same.</font> |
| - | * | + | **2. <font 16px/inherit;;inherit;;inherit>GM12878</font>** <font 16px/inherit;;inherit;;inherit>, reserved lot by Coriell: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | [[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]] | + | <font 16px/inherit;;inherit;;inherit>Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase</font> |
| - | * | + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/document/d/1wFZh5LbuMlXCAWMHKwk0aH0d7ZqLmaNnwDzh0mip_ko/edit?usp=sharing|Coriell Ordering Instructions (GM12878)]]</font> |
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]] | + | * [[https://docs.google.com/document/d/1kwYAUGBsuqs0JQ-TrrJ-pJ5MtyDWqoP9AwT-lw21ZVw/edit|Protocol to Order GM12878 Cells from Coriell]] (slides) |
| - | * | + | * //International Orders:// The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is customerservice@coriell.org[[customerservice@coriell.org|]] or call 1-856-757-4848. |
| - | [[https://drive.google.com/a/4dnucleome.org/file/d/0B1Uh14Y8H75dODNvdGFVZ2xsVjQ/view?usp=sharing|Material Transfer Agreement 4DN Addendum]] | + | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]09, 2018 |
| - | * | + | <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>Request a second vial:</font></font> |
| - | [[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]] | + | <font 16px/inherit;;inherit;;inherit>Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]). After you have send the letter the order procedure is the same.</font> |
| - | * | + | <font 16px/inherit;;inherit;;inherit>**3. IMR90**, reserved lot by ATCC: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]] | + | * <font 16px/inherit;;inherit;;inherit>[[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]]</font> |
| + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]]</font> | ||
| + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]]</font> | ||
| + | * <font 16px/inherit;;inherit;;inherit>[[https://drive.google.com/a/4dnucleome.org/file/d/0B1Uh14Y8H75dODNvdGFVZ2xsVjQ/view?usp=sharing|Material Transfer Agreement 4DN Addendum]]</font> | ||
| + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]]</font> | ||
| + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]]</font> | ||
| - | Process for international customers: | + | <font inherit/inherit;;#c0392b;;inherit><font 16px/inherit;;inherit;;inherit>Ordering process for customers (Updated process per ATCC on 3.4.2021):</font></font> |
| - | // Step 1:// 1. Consortium members will receive product availability email from ATCC with a fillable 4DN MTA Addendum | + | <font 16px/inherit;;inherit;;inherit>__Step 1:__</font> |
| - | [[https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing|https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing]] | + | <font 16px/inherit;;inherit;;inherit>1. Contact Stefan Brings ([[sbrings@atcc.org|]]) and he will verify that you are on the list of approved consortium members. If you are, Stefan Brings will provide you with the 4DN MTA Addendum. Once the addendum is signed, their Contracts team will review.</font> |
| - | 2.Members will sign the MTA and send it to Stefan Brings ([[http://sbrings@atcc.org/|sbrings@atcc.org]]). | + | <font 16px/inherit;;inherit;;inherit>[[https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing|https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing]]</font> |
| - | // Step 2:// 1. Stefan will send the[[https://drive.google.com/file/d/0B1Uh14Y8H75ddVhINllYbWRqLWc/view?usp=sharing|ATCC Order Form ]] through email. | + | <font 16px/inherit;;inherit;;inherit>2. ATCC Contracts will then provide Stefan Brings the approval to send an Order Form to the end user. This Order Form is to be completeed and returned to Stefan Brings.</font> |
| - | 2. 4DN Member will complete the order form and send it to [[sales@atcc.org|]] or Fax (703-365-2750) it for a quote (for shipping cost basically). | + | <font 16px/inherit;;inherit;;inherit>3. Once Stefan Brings have both the Order Form and the signed Addendum, he will provide these to his team for processing. He will then provide confirmation and estimated ship dates to the end user.</font> |
| - | 3. ATCC international order entry team will process the order. | + | <font 16px/inherit;;inherit;;inherit>*This entire process can take about 5-10 business days for review and regulatory release of material for shipping. (International orders are more complicated and will likely require permits for shipping and higher courier charges).</font> |
| - | Request a second vial: | + | <font 16px/inherit;;inherit;;inherit>__Step 2:__</font> |
| - | Request a letter of consent from Dr. David Gilbert ([[gilbert@bio.fsu.edu|]]) and send it by email to Stefan Brings from ATCC ([[sbrings@atcc.org|]]) | + | <font 16px/inherit;;inherit;;inherit>1. Stefan will send the[[https://drive.google.com/file/d/0B1Uh14Y8H75ddVhINllYbWRqLWc/view?usp=sharing|ATCC Order Form ]] through email.</font> |
| - | **4. HFF-hTert-clone 6 reserved lot from UMass**. AVAILABLE | + | <font 16px/inherit;;inherit;;inherit>2. 4DN Member will complete the order form and send it to [[sales@atcc.org|]] or Fax (703-365-2750) it for a quote (for shipping cost basically).</font> |
| - | The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. | + | <font 16px/inherit;;inherit;;inherit>3. ATCC international order entry team will process the order.</font> |
| - | The recipient PI will pay for the shipping of the cells using FedEx or UPS | + | <font inherit/inherit;;#c0392b;;inherit><font 16px/inherit;;inherit;;inherit>Request a second vial:</font></font> |
| - | To request the cells, please contact Johan Gibcus ([[johan.gibcus@umassmed.edu|]]) from UMass to set up MTA | + | <font 16px/inherit;;inherit;;inherit>Request a letter of consent from Dr. David Gilbert ([[gilbert@bio.fsu.edu|]]) and send it by email to Stefan Brings from ATCC ([[sbrings@atcc.org|]])</font> |
| - | * | + | <font 16px/inherit;;inherit;;inherit>**4. HFF-hTert-clone 6 reserved lot from UMass**. <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | [[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]] | + | <font 16px/inherit;;inherit;;inherit>The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge.</font> |
| - | **5. F121-9 hybrid CASTx129 Female Mouse ESC**: AVAILABLE | + | <font 16px/inherit;;inherit;;inherit>The recipient PI will pay for the shipping of the cells using FedEx or UPS</font> |
| - | Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions. | + | <font 16px/inherit;;inherit;;inherit>To request the cells, please contact Johan Gibcus ([[johan.gibcus@umassmed.edu|]]) from UMass to set up MTA</font> |
| - | * | + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]]</font> |
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]] | + | <font 16px/inherit;;inherit;;inherit>**5. F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | **NOTE**: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | + | <font 16px/inherit;;inherit;;inherit>Contact Dave Gilbert, or Takayo Sasaki for cell if any questions.</font> |
| - | **6. F123 hybrid CASTx129 Male Mouse ESC**: AVAILABLE | + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]</font> |
| - | Contact Bing Ren for vials or with questions. | + | <font 16px/inherit;;inherit;;inherit>**NOTE**: Shoji Takahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font> |
| - | * | + | <font 16px/inherit;;inherit;;inherit>**6. F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]] | + | <font 16px/inherit;;inherit;;inherit>Contact Bing Ren for vials or with questions.</font> |
| - | NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] | + | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]</font> |
| - | **7. HAP1 Cells:** AVAILABLE | + | <font 16px/inherit;;inherit;;inherit>NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font> |
| - | HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. | + | <font 16px/inherit;;inherit;;inherit>**7. HAP1 Cells:** <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> |
| - | The PI is responsible of the shipping cost | + | <font 16px/inherit;;inherit;;inherit>HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute.</font> |
| - | To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl]] | + | <font 16px/inherit;;inherit;;inherit>The PI is responsible of the shipping cost</font> |