Differences

This shows you the differences between two versions of the page.

--- 4dn:phase2:4dn_cell_repository_4 [2021/03/09 11:17]
oh [How to Order Tier 1 and Tier 2 Cells]
+++ 4dn:phase2:4dn_cell_repository_4 [2025/04/22 16:21] (current)
@@ Line 9: / Line 9: @@
 <font 16px/inherit;;inherit;;inherit>**<font inherit/inherit;;#c0392b;;inherit>__Eligibility information for PIs who are new to 4DN Cell Repository__</font>** </font>
-<font 14px/inherit;;inherit;;inherit>Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <[[gilbert@bio.fsu.edu|]]>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <[[oh@4ducleome.org|oh@4dnucleome.org]]>, a member of the 4DN Cell Line Working group.</font>
+All current Phase II PIs have been approved by the Phase II 4DN Cells WG to receive a single vial of each of our commercial repository stocked Tier1 cell lines, including: H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC). Additional vials will require separate approval from the Cells WG.
-<font 14px/inherit;;inherit;;inherit>**Content of your request letter**</font>
+**NOTE: **All PIs are reminded to stock the cells by 4DN standards and to follow the 4DN approved SOP for all cell lines. When data will be submitted to the DCIC, they will need to verify that the cells have been expanded in these conditions that are posted on the Cells WG Wiki page, and the appropriate metadata will need to be recorded at the time of cell harvest in order for datasets to receive the gold standard flag.
-<font 14px/inherit;;inherit;;inherit>In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. **Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.**</font>
+**[[https://drive.google.com/file/d/1Iq0dWUl6zLIQkYcAMFWMoaGFSPOLFxVK/view?usp=sharing|Copy of the consent letter for Phase II PIs]]**
-<font 14px/inherit;;inherit;;inherit>**Review of your letter**</font>
-<font 14px/inherit;;inherit;;inherit>After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the “current PI list who have requested this cell line” on 4DN wiki.</font>
-=====   =====
@@ Line 37: / Line 31: @@
 [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]]
-<font inherit/inherit;;#c0392b;;inherit><font 16px/inherit;;inherit;;inherit>**ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?**</font></font>- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]] of who is engineering what so as not to duplicate effort or work together!
@@ Line 68: / Line 60: @@
   * //International Orders://  The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is customerservice@coriell.org[[customerservice@coriell.org|]] or call 1-856-757-4848.
   * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]09, 2018
 <font 16px/inherit;;inherit;;inherit><font inherit/inherit;;#c0392b;;inherit>Request a second vial:</font></font>
@@ Line 83: / Line 75: @@
   * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]]</font>
-<font inherit/inherit;;#c0392b;;inherit><font 16px/inherit;;inherit;;inherit>Process for international customers:</font></font>
+<font inherit/inherit;;#c0392b;;inherit><font 16px/inherit;;inherit;;inherit>Ordering process for customers (Updated process per ATCC on 3.4.2021):</font></font>
 <font 16px/inherit;;inherit;;inherit>__Step 1:__</font>
-<font 16px/inherit;;inherit;;inherit>1. Consortium members will receive product availability email from ATCC with a fillable 4DN MTA Addendum</font>
+<font 16px/inherit;;inherit;;inherit>1. Contact Stefan Brings ([[sbrings@atcc.org|]]) and he will verify that you are on the list of approved consortium members. If you are, Stefan Brings will provide you with the 4DN MTA Addendum. Once the addendum is signed, their Contracts team will review.</font>
 <font 16px/inherit;;inherit;;inherit>[[https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing|https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing]]</font>
-<font 16px/inherit;;inherit;;inherit>2.Members will sign the MTA and send it to Stefan Brings ([[http://sbrings@atcc.org/|sbrings@atcc.org]]).</font>
+<font 16px/inherit;;inherit;;inherit>2. ATCC Contracts will then provide Stefan Brings the approval to send an Order Form to the end user. This Order Form is to be completeed and returned to Stefan Brings.</font>
+<font 16px/inherit;;inherit;;inherit>3. Once Stefan Brings have both the Order Form and the signed Addendum, he will provide these to his team for processing. He will then provide confirmation and estimated ship dates to the end user.</font>
+<font 16px/inherit;;inherit;;inherit>*This entire process can take about 5-10 business days for review and regulatory release of material for shipping. (International orders are more complicated and will likely require permits for shipping and higher courier charges).</font>
 <font 16px/inherit;;inherit;;inherit>__Step 2:__</font>
@@ Line 117: / Line 113: @@
 <font 16px/inherit;;inherit;;inherit>**5. F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font>
-<font 16px/inherit;;inherit;;inherit>Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.</font>
+<font 16px/inherit;;inherit;;inherit>Contact Dave Gilbert, or Takayo Sasaki for cell if any questions.</font>
   * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]</font>
-<font 16px/inherit;;inherit;;inherit>**NOTE**: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font>
+<font 16px/inherit;;inherit;;inherit>**NOTE**: Shoji Takahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font>
 <font 16px/inherit;;inherit;;inherit>**6. F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font>
@@ Line 137: / Line 133: @@
 <font 16px/inherit;;inherit;;inherit>The PI is responsible of the shipping cost</font>

4D Nucleome Network Wiki

User Tools

Site Tools

Differences