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4dn:phase2:4dn_cell_repository_4
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4dn:phase2:4dn_cell_repository_4 [2021/06/09 11:26] david_gilber [Material Transfer Agreements (MTAs) - DISCLAIMER] |
4dn:phase2:4dn_cell_repository_4 [2025/04/22 16:21] (current) |
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| <font 16px/inherit;;inherit;;inherit>**<font inherit/inherit;;#c0392b;;inherit>__Eligibility information for PIs who are new to 4DN Cell Repository__</font>** </font> | <font 16px/inherit;;inherit;;inherit>**<font inherit/inherit;;#c0392b;;inherit>__Eligibility information for PIs who are new to 4DN Cell Repository__</font>** </font> | ||
| - | <font 14px/inherit;;inherit;;inherit>Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <[[gilbert@bio.fsu.edu|]]>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <[[oh@4ducleome.org|oh@4dnucleome.org]]>, a member of the 4DN Cell Line Working group.</font> | + | All current Phase II PIs have been approved by the Phase II 4DN Cells WG to receive a single vial of each of our commercial repository stocked Tier1 cell lines, including: H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC). Additional vials will require separate approval from the Cells WG. |
| - | <font 14px/inherit;;inherit;;inherit>**Content of your request letter**</font> | + | **NOTE: **All PIs are reminded to stock the cells by 4DN standards and to follow the 4DN approved SOP for all cell lines. When data will be submitted to the DCIC, they will need to verify that the cells have been expanded in these conditions that are posted on the Cells WG Wiki page, and the appropriate metadata will need to be recorded at the time of cell harvest in order for datasets to receive the gold standard flag. |
| - | <font 14px/inherit;;inherit;;inherit>In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. **Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.**</font> | + | **[[https://drive.google.com/file/d/1Iq0dWUl6zLIQkYcAMFWMoaGFSPOLFxVK/view?usp=sharing|Copy of the consent letter for Phase II PIs]]** |
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| - | <font 14px/inherit;;inherit;;inherit>**Review of your letter**</font> | + | |
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| - | <font 14px/inherit;;inherit;;inherit>After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the “current PI list who have requested this cell line” on 4DN wiki.</font> | + | |
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| - | ===== ===== | + | |
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| [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | ||
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| - | <font 24px/inherit;;inherit;;inherit>**Are You Engineering or Have you Engineered Any of the 4DN Cell Lines or Would You Like to Know What Engineered Lines Have Been or are Being Made?**</font> | ||
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| - | **START** by looking at this spread sheet of completed and planned cell lines: [[https://docs.google.com/spreadsheets/d/19cbxd_KFPNIa-Op8MCxH7ViEGlqMRxftrY73HCbikIA/edit#gid=0|Engineered Cell Lines]] | ||
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| - | **IF YOU ARE PLANNING TO OR ARE IN THE PROCESS OF MAKING A LINE: **Please let the cells working group know (contacts at the top of the page) and please discuss your plans on this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]] of who is engineering what so as not to duplicate effort or to work together! If you cannot figure out how to post to the forum, we will start it for you when you email us. | ||
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| - | **IF YOU HAVE AN ENGINEERED LINE YOU ARE WILLING TO SHARE:** please let the cells working group know (contacts at the top of this page) and we will post it to the spread sheet above. | ||
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| <font 16px/inherit;;inherit;;inherit>**5. F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> | <font 16px/inherit;;inherit;;inherit>**5. F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> | ||
| - | <font 16px/inherit;;inherit;;inherit>Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.</font> | + | <font 16px/inherit;;inherit;;inherit>Contact Dave Gilbert, or Takayo Sasaki for cell if any questions.</font> |
| * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]</font> | * <font 16px/inherit;;inherit;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]</font> | ||
| - | <font 16px/inherit;;inherit;;inherit>**NOTE**: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font> | + | <font 16px/inherit;;inherit;;inherit>**NOTE**: Shoji Takahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]]</font> |
| <font 16px/inherit;;inherit;;inherit>**6. F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> | <font 16px/inherit;;inherit;;inherit>**6. F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#c0392b;;inherit>AVAILABLE</font></font> | ||
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| <font 16px/inherit;;inherit;;inherit>The PI is responsible of the shipping cost</font> | <font 16px/inherit;;inherit;;inherit>The PI is responsible of the shipping cost</font> | ||