==== Omics Data Standards WG - Minutes 03-13-2017 ==== |~~TABLE_CELL_WRAP_START~~ Repli-seq \__ 1.-Resolution of replication timing experiments: Biological limitation Peaks within domains Boundary resolution \__ 4DN Goal: define what resolution means \__ 2.- E/L method Different cell lines have different data qualities and resolutions as well \__ DNA labeling: BrdU vs EdU. PFA Fixation affects the map Quality controls list \__ Variation in sized in a typical experiment (for QC) \__ 2.- Processing E/L method \__ Shape recognition for “ripples”, are those discontinuities in replication domains? \__ To what extent the resolution is limited by cell-to-cell variation instead of technology. Combination of single cells experiments are needed \__ 3. Repli-chip vs Repli-seq \__ In most of the cases Repli-chip is adequate but not all cases. \__ 4. Repli-seq biases and QC \__ Are there additional information that will be useful to evaluate Repli-seq data? Those data can be included when depositing Repli-seq data. A second session can be scheduled to discuss about these. ~~TABLE_CELL_WRAP_STOP~~|