Table of Contents

Imaging Data Standards

The mailing list for the working group has been changed. Please use image@4dnucleome.org from now on as the new mailing list address.

IMAGING DATA STANDARDS: ESTABLISH STANDARDS FOR DATA FORMATS AND QUALITY CONTROL

Co-Chairs:

Year 4: Rob Singer and David Grunwald

Year 3: Rob Singer, Joan Ritland and David Grunwald

Year 2: Rob Singer, Joan Ritland .

Year 1: Mark Ellisman, Xavier Darzacq

DATA UPLOAD IS LIVE

(Since March, 2018)

Upload of imaging data to the 4DN portal is live. Please let us know if you have Imaging data gathered with 4DN funding and you are not on this list:https-3A__docs.google.com_spreadsheets_d_14Msheyhc-2Du8KnU3R5W8Ow-2DuSPyoMKlWBsvJYAsRTxVg_edit-3Fusp-3Dsharing.

This link gives information on the upload process from DCIC:180306_dcic_data_upload.pdf

Imaging WG Meeting WebEx Information

1st Monday of every month, 12:00 pm PT (3:00 pm ET)
Meeting Link:
https://4dnucleome.webex.com/4dnucleome/j.php?MTID=m033f3efc842cb3821d91cfbf9a125375
Meeting number: 806 955 362
Meeting password: KvTsi3bA
Host key: 927479
Audio Connection: 1-415-655-0002 US TOLL
Access code: 806 955 362

WEBEX EMERGENCY CONTACT INFORMATION:

KATE RIVERA

858-822-1626 (OFFICE)

Mission / Objectives

Year 3 goals:

Year 2 goals

Year 1 goals

*SCOPE* The 4D Nucleome Initiative investigators efforts will result in production of a variety of different types of datasets obtained by imaging the nuclei of single cells with multiple methodologies. An immediate objective of this working group is to identify the image data types; to develop a format for each and to keep a working list of the nature of these datasets. The group will discuss these topics and determine our course of action. We hope to determine how these data would be archived and retrieved by other 4DN groups, and the larger community of biomedical investigators. We will also work to implement, among the imaging groups of the 4D nucleome, practices that enable a suitable data sharing strategy – one that might resemble that of the genomics field (eg., data deposition prior to publication and shared upon publication). *TASKS* Our first task will be to define the list of datasets and data types that are being generated under 4DN support and assemble a small working group with sufficient expertise to work out the details required to properly annotate and archive all of them. After our meeting we will collect information about these datasets from the different groups.

- For each type of data we consider how to establish bridges that link to data types of the other working groups

Meeting notes

  1. February 2016FEB MEETING NOTES
  2. April 2016 -APRIL MEETING NOTES
  3. February 2017Feb 2017 minutes
  4. June 2017 minutesJune 2017 minutes
  5. July 2017 minutesJuly 2017 minutes
  6. August 2017 minutesAugust Imaging Minutes.docx
  7. November 2017 minutes: Jason Swedlow presentation on Euro BioImaging image curation pipelines
  8. January 2018 minutes : LINCspresentation on image curation and integration with other data types
  9. No Feb meeting
  10. March 6, 2018.DCIC and Gianluca Pegora presentation on progress with Misteli HIP FISH data upload. (minutes, recordings)
  11. March 21, 2018.Allen Institute for Cell Sciences presented their approaches to image data management and machine learning for predicting nuclear structure. (minutes,recordings)
  12. April 3, 2018 Anders Hansen (Darzacq lab) presented their SPT data and progress on SPT data annotation and upload. IWG approves upload of trajectory data only at this time - raw and analyzed data formats will be defined in the future. Caterina Strombio, a non-4DN member who has been designing tools for managing and analyzing SPT data at UMASS, sat in on this call. (recordings, Anders Hansen SPT format ppt, minutes)
  13. April 18, 2018. Charles Kenworthy (Coleman/Singer) presentedSPTdatafrom theirnew BioRxivpaper. Kenworthy ppt. Kenworthy movie. The SPT metadata format presented at the April 3rd meeting was approved . There were no commentson the drafts Anders circulated. Caterina Strombio sat in onthis call also. (recordings, minutes)
  14. May 1,2018Tristin Liu (Ren lab) presents SOX2 imaging data. This is one of the joint analysis common gene targets (recordings,minutes)
  15. May 16, 2018.Sofi Quinodoz (Guttman Lab) will present imaging data from their SPRITE paper (recordings)
  16. June 5, 2018 Organizational MeetingJune 4, 2018 minutes
  17. June 20, 2018 Raja Ghosh (Liphardt lab) presents SPT data and progress on SPT data annotation and upload.(recording)
  18. August 8 at 1PM Pacific - note time change - David Baddeley will present approaches to image curation.(recording)
  19. September 4, 2018 - (recording), agenda
  20. September 19, 2018. Peter Wang (presenter), AGENDA, Recording, Minutes,
  21. October 2, 2018 - Dr. Bing Ren, Dr. William Noble and Dr. Susanne Rafelski will present. (recording)
  22. October 17, 2018 - Ting Wu (presenter), (PP presentation), (recording)
  23. November 6, 2018 - Paul Kaufman and Gianluca Pegoraro (presenters - 20 minutes shorter), talk discussion (20 minutes), Subgroup Omics to Imaging report (20 minutes), AGENDA, (recording)
  24. November 28, 2018 - (recording)
  25. December 19, 2018 - (recording)
  26. January 16, 2019 - CANCELLED
  27. February 4 , 2019 - (recording)
  28. May 6, 2019 - agenda, recording
  29. June 3, 2019 - CANCELLED
  30. July 1, 2019 - agenda - CANCELLED
  31. August 5, 2019 - agenda, recording
  32. September 9, 2019 - CANCELLED
  33. October 7, 2019 - recording
  34. November 4, 2019 - slides, recording (Talk by Max Huisman (Grunwald lab) MetaMax Generation 3 – What you would like to know about your microscope that will stay hidden from meta data), recording
  35. December 2, 2019 - CANCELLED
  36. January 6, 2020 - CANCELLED
  37. February 3, 2020 - recording
  38. March 2, 2020 - CANCELLED
  39. April 6, 2020 - CANCELLED
  40. May 4, 2020 - Agenda, Recording link
  41. June 1, 2020 - Agenda, Recording link

2017 Annual Meeting

Documents

Metadata documents, October 2017:

Guidance for uploading DNA FISH data to DCIC Guidance to DNA FISH labs.docx

Open the docs below with Google docs to be able to add your comments:

Groudine G4 TetO gene trap library

Data

Groudine G4 TetO gene trap library

NUP107 A647 images:


Ries/Ellenberg, EMBL

Ritland/Groudine, FredHutch

Singer Lab, Einstein and Janelia

Bewersdorf Lab, Yale

Zipfel, Cornell

Peltier/Ellisman

Members

Insert spreadsheet members

Mailing list

The mailing list to use for communication in this Working Group is image@4dnucleome.org. Emails sent there will go to everyone on the list. Replying to an email from the list will also send it to everyone in the list.

Calendar