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| - | ====== 4DN Cell Repository ====== | + | ==== 4DN Cell Repository ==== |
| - | <font 22px/inherit;;inherit;;inherit>This page describes the ordering process for the established cell lines of the 4DN Cell Repository. The established cell lines specifically refer to the cell lines that have been approved by the Cell Line WG, 4DN steering committee, NIH, and have been ordered by OH or created by 4DN appointed groups.</font> | + | |~~TABLE_CELL_WRAP_START~~<WRAP> |
| - | Please note that there are additional cell lines that are under discussion by the Cell Line WG. | + | This page describes the ordering process for the established cell lines of the 4DN Cell Repository. The established cell lines specifically refer to the cell lines that have been approved by the Cell Line WG, 4DN steering committee, NIH, and have been ordered by OH or created by 4DN appointed groups.~~CKG_TABLE_NBSP~~ |
| - | **<font 20px/inherit;;#2980b9;;inherit>Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC</font>** | + | ~~CKG_TABLE_NBSP~~ |
| - | **<font 20px/inherit;;#e74c3c;;inherit>Eligibility information for PIs who are new to 4DN Cell Repository</font>** | + | Please note that there are additional cell lines that are under discussion by the Cell Line WG.~~CKG_TABLE_NBSP~~ |
| - | **Please see this page for the available cell lines and the ordering process.** Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <gilbert@bio.fsu.edu>, co-chair of Cell Line Working group, and copy Kathia Zaleta-Rivera <kzaletar@eng.ucsd.edu>, a member of the 4DN Cell Line Working group. | + | ~~CKG_TABLE_NBSP~~ |
| - | <font 18px/arial, sans-serif;;#222222;;inherit>**Content of your request letter**</font> | + | <font inherit/inherit;;#2980b9;;inherit><font 16px/inherit;;inherit;;inherit>**Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC**</font></font> |
| - | <font inherit/arial, sans-serif;;#222222;;inherit>In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. **Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy. **</font> | + | ~~CKG_TABLE_NBSP~~ |
| - | <font 18px/arial, sans-serif;;#222222;;inherit>**Review of your letter**</font> | + | __<font inherit/inherit;;#e74c3c;;inherit><font 16px/inherit;;inherit;;inherit>**Eligibility information for PIs who are new to 4DN Cell Repository~~CKG_TABLE_NBSP~~**</font></font>__ |
| - | <font inherit/arial, sans-serif;;#222222;;inherit>After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the "current PI list who have requested this cell line" on 4DN wiki.</font> | + | ~~CKG_TABLE_NBSP~~ |
| - | **<font 20px/inherit;;inherit;;inherit>Consent for lines isolated prior to January 15, 2015 - DISCLAIMER</font>** | + | For access to 4DN Cell Repository cells, please see~~CKG_TABLE_NBSP~~[[https://wiki.4dnucleome.org/4dn:4dn_cell_repository|4DN Cell Repository wiki page]]~~CKG_TABLE_NBSP~~for the available cell lines and the ordering process. Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <[[gilbert@bio.fsu.edu|]]>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <[[oh@4ducleome.org|oh@4dnucleome.org]]>, a member of the 4DN Cell Line Working group. |
| + | |||
| + | ~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | <font 14px/inherit;;inherit;;inherit>**Content of your request letter**</font> | ||
| + | |||
| + | In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells.~~CKG_TABLE_NBSP~~**Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.** | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | <font 14px/inherit;;inherit;;inherit>**Review of your letter**</font> | ||
| + | |||
| + | After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the "current PI list who have requested this cell line" on 4DN wiki.~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | ===== Consent for lines isolated prior to January 15, 2015 - DISCLAIMER ===== | ||
| Posting of public data from specimens collected before January 15, 2015 is the responsibility of and must be approved by each PIs institution. Additional questions about Consent should be addressed by individual PIs with their institutions. | Posting of public data from specimens collected before January 15, 2015 is the responsibility of and must be approved by each PIs institution. Additional questions about Consent should be addressed by individual PIs with their institutions. | ||
| - | [[https://gds.nih.gov/pdf/GDS_Clinical_Specimen_Certification.pdf|Sample Institutional Certification Form for Public sharing of data from specimens collected before January 15, 2015 that lack consent]] | + | <font 14px/inherit;;inherit;;inherit>[[https://www.google.com/url?q=https%3A%2F%2Fgds.nih.gov%2Fpdf%2FGDS_Clinical_Specimen_Certification.pdf&sa=D&sntz=1&usg=AFQjCNHwXok1gsl8Oeae1SfafOmZEjFbiQ|Sample Institutional Certification Form for Public sharing of data from specimens collected before January 15, 2015 that lack consent]]</font> |
| - | [[https://gds.nih.gov/13faqs_gds.html|NIH page of FAQs for Genomic Data Sharing Policy]] | + | [[https://www.google.com/url?q=https%3A%2F%2Fgds.nih.gov%2F13faqs_gds.html&sa=D&sntz=1&usg=AFQjCNGhy8ba4aSIvlyY5c0OzD-D9XYwYA|NIH page of FAQs for Genomic Data Sharing Policy]]: |
| - | [[https://www.research.fsu.edu/research-compliance/genomic-data-sharing/|(EXAMPLE) Florida State University RESEARCH COMPLIANCE page]] | + | [[https://www.google.com/url?q=https%3A%2F%2Fwww.research.fsu.edu%2Fresearch-compliance%2Fgenomic-data-sharing%2F&sa=D&sntz=1&usg=AFQjCNHk0RU-biKd5flFAM6fw6fjXuiGGA|(EXAMPLE) Florida State University RESEARCH COMPLIANCE page]] |
| - | <font 20px/inherit;;inherit;;inherit>**Material Transfer Agreements (MTAs) - DISCLAIMER**</font> | + | ===== Material Transfer Agreements (MTAs) - DISCLAIMER ===== |
| - | <font inherit/Arial;;inherit;;inherit>MTAs are the responsibility of each PI and their institution and are to be negotiated separately between each institution and the distributor. Additional questions about MTAs should be addressed by individual PIs with their institutions. The document below is a list of salient points from the MTAs between the OH (UCSD) and the three major providers, provided to 4DN PIs for your convenience as a courtesy and not to be considered a binding document.</font> | + | <font 14px/inherit;;inherit;;inherit>MTAs are the responsibility of each PI and their institution and are to be negotiated separately between each institution and the distributor. Additional questions about MTAs should be addressed by individual PIs with their institutions. The document below is a list of salient points from the MTAs between the OH (UCSD) and the three major providers, provided to 4DN PIs for your convenience as a courtesy and not to be considered a binding document.</font> |
| [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | [[https://docs.google.com/document/d/1hGAQ2_72sqkzdlCQWHLRzCUOtTqJs09KxrAdevRVlDU/edit|Readers Digest List of MTA Conditions Condensed by the OH]] | ||
| - | <font inherit/inherit;;#e74c3c;;inherit>**ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?**</font>- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]] of who is engineering what so as not to duplicate effort or work together! | + | **<font inherit/inherit;;#e74c3c;;inherit><font 16px/inherit;;inherit;;inherit>ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?</font></font>**- please see this [[https://groups.google.com/a/4dnucleome.org/forum/#!forum/4dn-cell-eng|discussion group]]~~CKG_TABLE_NBSP~~of who is engineering what so as not to duplicate effort or work together! |
| ===== How to Order Tier 1 and Tier 2 Cells ===== | ===== How to Order Tier 1 and Tier 2 Cells ===== | ||
| - | <font inherit/inherit;;#e74c3c;;inherit>**IMPORTANT! **</font>The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. | + | <font inherit/inherit;;#e74c3c;;inherit>**IMPORTANT!**</font>~~CKG_TABLE_NBSP~~The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. |
| - | __**Before thawing the cells**__ | + | <font 14px/inherit;;inherit;;inherit>**Before thawing the cells**</font>\\ \\ <font inherit/inherit;;#e74c3c;;inherit>**Read the finalized SOP for your cell line from**</font> ~~CKG_TABLE_NBSP~~[[https://wiki.4dnucleome.org/4dn:working_groups:cell_lines_and_samples|Cell lines WG wiki page]].~~CKG_TABLE_NBSP~~The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. |
| - | <font inherit/inherit;;#e74c3c;;inherit>Read the finalized SOP for your cell line from the Cell lines WG wiki page.</font>The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. | + | **<font inherit/inherit;;#e74c3c;;inherit>DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!</font>** |
| - | <font inherit/inherit;;#e74c3c;;inherit>**DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!**</font> | + | \\ **1.~~CKG_TABLE_NBSP~~Human embryonic stem cells H1**, by WiCell.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | <font 20px/inherit;;inherit;;inherit>**1.** **Human embryonic stem cells H1**, by WiCell. AVAILABLE</font> | + | * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN%20WiCell%20Ordering%20Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]]~~CKG_TABLE_NBSP~~([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]])~~CKG_TABLE_NBSP~~August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost. |
| - | * [[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg/4DN WiCell Ordering Process.pdf?attredirects=0&d=1|WICell Ordering Process [WA01 (H1)]]] ([[https://docs.google.com/presentation/d/1LEfB61kE1Sq4Np-2qMNpQK_QHW5yYADEntIeuYAYvPA/edit#slide=id.g17d5e478f2_0_47|supplementary slides]]) August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost. | ||
| * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]] | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0ODAzY2NjYzYzODliNDEz|WiCell International Ordering Process (H1)]] | ||
| <font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | <font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | ||
| - | Request a letter of authorization from the OH (<kzaletar@eng.ucsd.edu>) and send it by email to Deborah Requesens from Coriell (<drequesens@coriell.org>). After you have sent the letter, the order procedure is the same. | + | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]]) and send it by email to Tennielle Ludwig from WiCell ([[tludwig@wicell.org|]]). After you have send the letter the order procedure is the same. |
| - | <font 20px/inherit;;inherit;;inherit>**2. GM12878, **reserved lot by Coriell: AVAILABLE</font> | + | **2.~~CKG_TABLE_NBSP~~GM12878**, reserved lot by Coriell:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase. | + | ~~CKG_TABLE_NBSP~~Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase |
| * [[https://docs.google.com/document/d/1wFZh5LbuMlXCAWMHKwk0aH0d7ZqLmaNnwDzh0mip_ko/edit?usp=sharing|Coriell Ordering Instructions (GM12878)]] | * [[https://docs.google.com/document/d/1wFZh5LbuMlXCAWMHKwk0aH0d7ZqLmaNnwDzh0mip_ko/edit?usp=sharing|Coriell Ordering Instructions (GM12878)]] | ||
| - | * [[https://docs.google.com/document/d/1kwYAUGBsuqs0JQ-TrrJ-pJ5MtyDWqoP9AwT-lw21ZVw/edit|Protocol to Order GM12878 Cells from Coriell]] (slides) | ||
| - | * __International Orders__: The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is <customerservice@coriell.org> or call 1-856-757-4848 . | ||
| - | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March 09, 2018]] | ||
| - | <font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | + | * [[https://docs.google.com/document/d/1kwYAUGBsuqs0JQ-TrrJ-pJ5MtyDWqoP9AwT-lw21ZVw/edit|Protocol to Order GM12878 Cells from Coriell]]~~CKG_TABLE_NBSP~~(slides) |
| - | Request a letter of authorization from the OH (<kzaletar@eng.ucsd.edu>) and send it by email to Deborah Requesens from Coriell (<drequesens@coriell.org>). After you have sent the letter, the order procedure is the same. | + | * <font inherit/inherit;;#3498db;;inherit>__International Orders:__</font>~~CKG_TABLE_NBSP~~The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is customerservice@coriell.org[[customerservice@coriell.org|]] or call <font inherit/inherit;;#3498db;;inherit>1-856-757-4848</font>. |
| - | <font 20px/inherit;;#e74c3c;;inherit>**3. IMR90**, reserved lot by ATCC: AVAILABLE</font> | + | * <font inherit/inherit;;#3498db;;inherit>[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2YWZjOTg2Y2U1ODIxZThi|Current PI List that has requested GM12878 Cells as March]]</font><font inherit/inherit;;#3498db;;inherit>09, 2018</font> |
| - | * [[https://docs.google.com/document/d/1_mZaHK2e_5W3aREFt-NaA9G99yJgnyzt8cDzfxPwaug/edit?usp=sharing|ATCC guide to online ordering (IMR90)]] | + | ~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> |
| - | * [[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]] | + | |
| - | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]] | + | |
| - | * [[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Material Transfer Agreement 4DN Addendum]] | + | |
| - | * [[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]] | + | |
| - | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]] | + | |
| - | <font 18px/inherit;;#e74c3c;;inherit>Process for international customers</font> | + | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]])~~CKG_TABLE_NBSP~~and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]).~~CKG_TABLE_NBSP~~After you have send the letter the order procedure is the same. |
| - | Step 1: | + | ~~CKG_TABLE_NBSP~~ |
| - | * 1. Consortium members will receive product availability email from ATCC with a [[https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing|fillable 4DN MTA Addendum]] | + | **3.~~CKG_TABLE_NBSP~~IMR90**, reserved lot by ATCC:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | * 2. Members will sign the MTA and send it to Angela Miller (<amiller@atcc.org>) | + | |
| - | Step 2: | + | * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]] |
| - | * 1. Angela will send the [[https://drive.google.com/file/d/0B1Uh14Y8H75ddVhINllYbWRqLWc/view?usp=sharing|ATCC Order Form]] through email. | + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]] |
| - | * 2. 4DN Member will complete the order form and send it to <sales@atcc.org> or Fax (703-365-2750) it for a quote (for shipping cost basically). | + | |
| - | * 3. ATCC international order entry team will process the order. | + | |
| - | <font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]] |
| + | |||
| + | * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/a/4dnucleome.org/file/d/0B1Uh14Y8H75dODNvdGFVZ2xsVjQ/view?usp=sharing|Material Transfer Agreement 4DN Addendum]]~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]] | ||
| + | |||
| + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo2MmM2OTcyMWVhZTRjYmVl|Current PI List that has Requested IMR90 cells_March 24, 2017]] | ||
| + | |||
| + | <font inherit/inherit;;#e74c3c;;inherit>Process for international customers:</font> | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~__ Step 1:__ | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~1. Consortium members will receive product availability email from ATCC with a fillable 4DN MTA Addendum | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~[[https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing|https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing]] | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~2.Members will sign the MTA and send it to Stefan Brings ([[http://sbrings@atcc.org|sbrings@atcc.org]]). | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~__ Step 2:__ | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~1. Stefanwill send the[[https://drive.google.com/file/d/0B1Uh14Y8H75ddVhINllYbWRqLWc/view?usp=sharing|ATCC Order Form ]]~~CKG_TABLE_NBSP~~through email. | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~2. 4DN Member will complete the order form and send it to [[sales@atcc.org|]]~~CKG_TABLE_NBSP~~or Fax (703-365-2750) it for a quote (for shipping cost basically). | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~3. ATCC international order entry team will process the order. | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~Request a letter of consent from Dr. David Gilbert ([[gilbert@bio.fsu.edu|]])~~CKG_TABLE_NBSP~~and send it by email to Stefan Brings from ATCC ([[sbrings@atcc.org|]]) | ||
| + | |||
| + | **4.~~CKG_TABLE_NBSP~~HFF-hTert-clone 6 reserved lot from UMass**.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font>~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~The recipient PI will pay for the shipping of the cells using FedEx or UPS | ||
| + | |||
| + | ~~CKG_TABLE_NBSP~~To request the cells, please contact Johan Gibcus ([[johan.gibcus@umassmed.edu|]]) from UMass to set up MTA~~CKG_TABLE_NBSP~~ | ||
| + | |||
| + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]] | ||
| + | |||
| + | **5.~~CKG_TABLE_NBSP~~F121-9 hybrid CASTx129 Female Mouse ESC**: <font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> | ||
| + | |||
| + | Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.~~CKG_TABLE_NBSP~~ | ||
| - | Request a letter of consent from Dr. David Gilbert (<gilbert@bio.fsu.edu>) and send it by email to Angela Miller from ATCC (<amiller@attcc.org>) | + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]~~CKG_TABLE_NBSP~~ |
| - | <font 20px/inherit;;#e74c3c;;inherit>**4. HFF-hTert-clone 6**, reserved lot from UMass. AVAILABLE</font> | + | ~~CKG_TABLE_NBSP~~**NOTE**: ShojiTakahashi~~CKG_TABLE_NBSP~~is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] |
| - | The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. The recipient PI will pay for the shipping of the cells using FedEx or UPS. To request the cells, please contact Johan Gibcus (<johan.gibcus@umassmed.edu>) from UMass to set up MTA. | + | **6.~~CKG_TABLE_NBSP~~F123 hybrid CASTx129 Male Mouse ESC**: <font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | * [[https://docs.google.com/document/d/159J54l-WSRWFx-SVwqyCWvWARr0WM1aVAApblnLh_P0/edit|Ordering Process]] | + | Contact Bing Ren for vials or with questions.~~CKG_TABLE_NBSP~~ |
| - | <font 20px/inherit;;#e74c3c;;inherit>**5. F121-9 hybrid CASTx129 Female Mouse ESC:**AVAILABLE</font> | + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]]~~CKG_TABLE_NBSP~~ |
| - | Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions. | + | ~~CKG_TABLE_NBSP~~NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: [[takahash@wi.mit.edu|]] |
| - | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]] | + | **7. HAP1 Cells:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | NOTE: Shoji Takahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: <takahash@wi.mit.edu> | + | ~~CKG_TABLE_NBSP~~HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. |
| - | <font 20px/inherit;;#ff0000;;inherit>**6. F123 hybrid CASTx129 Male Mouse ESC:** AVAILABLE</font> | + | ~~CKG_TABLE_NBSP~~The PI is responsible of the shipping cost |
| - | Contact Bing Ren for vials or with questions. | + | ~~CKG_TABLE_NBSP~~To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl ]] |
| - | * [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoxOGM5MmQwYWRkODNmZTYz|Simple Letter Agreement (SLA) for CASTx129]] | + | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] |
| - | NOTE: Shoji Takahashiis in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: <takahash@wi.mit.edu> | + | **8.~~CKG_TABLE_NBSP~~Human embryonic stem cells H9 (female)**, by WiCell.~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | <font 20px/inherit;;#e74c3c;;inherit>**7. HAP1 Cells:**AVAILABLE</font> | + | ~~CKG_TABLE_NBSP~~Obtain from WiCell, use SOP listed on Cells Wiki |
| - | HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. The PI is responsible of the shipping cost. To order the cells and sign MTA contact Dr. Bas van Steensel by email: <b.v.steensel@nki.nl> | + | **9. hTERT-RPE:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | * [[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] | + | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**8. Human embryonic stem cells H9 (female)**, by WiCell. AVAILABLE</font> | + | **10. K562:~~CKG_TABLE_NBSP~~** <font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from WiCell, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**9. hTERT-RPE **AVAILABLE</font> | + | **11. HEK293:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from ATCC, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**10. K562 **AVAILABLE</font> | + | **12.~~CKG_TABLE_NBSP~~WTC-11**:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from ATCC, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Obtain from Coriell, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**11. HEK293 **AVAILABLE</font> | + | **13. U2OS:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from ATCC, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Obtain from ATCC, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**12. WTC-11 **AVAILABLE</font> | + | **14. JM8.N4**: <font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from Coriell, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Obtain from KOMP, use SOP listed on Cells Wiki |
| - | <font 20px/inherit;;#e74c3c;;inherit>**13. U2OS **AVAILABLE</font> | + | **15. G4 mESC:**~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> |
| - | Obtain from ATCC, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~Contact Carol Ware from the ISCRM-University of Washinton for G4 mESC distribution [[cware@u.washington.edu|]]~~CKG_TABLE_NBSP~~ |
| - | <font 20px/inherit;;#e74c3c;;inherit>**14. JM8.N4 **AVAILABLE</font> | + | ~~CKG_TABLE_NBSP~~Execute and sign [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo3NDAxNzFmYmYxMTJmZmZh|Material Transfer Agreement (MTA]]) with Mt. Sinai contact Michael Hanna by email:~~CKG_TABLE_NBSP~~[[http://hanna@lunenfeld.ca|hanna@lunenfeld.ca]] |
| - | Obtain from KOMP, use SOP listed on Cells Wiki | + | ~~CKG_TABLE_NBSP~~The PI is responsible for the shipping cost |
| - | <font 20px/inherit;;#e74c3c;;inherit>**15. G4 mESC **AVAILABLE</font> | + | ~~CKG_TABLE_NBSP~~Use SOP listed on Cells Wiki |
| - | Contact Carol Ware from the ISCRM -University of Washington for G4 mESC distribution <cware@u.washington.edu>. Execute and sign [[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo3NDAxNzFmYmYxMTJmZmZh|Material Transfer Agreement (MTA)]] with Mt. Sinai contact Michael Hanna by email: <hanna@lunenfeld.ca>. The PI is responsible for the shipping cost. Use SOP listed on Cells Wiki. | + | ~~CKG_TABLE_NBSP~~ |
| + | </WRAP>~~TABLE_CELL_WRAP_STOP~~| | ||
| + | |~~CKG_TABLE_NBSP~~| | ||
4dn/phase1/4dn_cell_repository.1550540142.txt.gz · Last modified: 2025/04/22 16:21 (external edit)