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4DN Cell Repository
This page describes the ordering process for the established cell lines of the 4DN Cell Repository. The established cell lines specifically refer to the cell lines that have been approved by the Cell Line WG, 4DN steering committee, NIH, and have been ordered by OH or created by 4DN appointed groups.
Please note that there are additional cell lines that are under discussion by the Cell Line WG.
Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC
Eligibility information for PIs who are new to 4DN Cell Repository
For access to 4DN Cell Repository cells, please see 4DN Cell Repository wiki page for the available cell lines and the ordering process. Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <gilbert@bio.fsu.edu>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <oh@4dnucleome.org>, a member of the 4DN Cell Line Working group.
Content of your request letter In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.
Review of your letter After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the “current PI list who have requested this cell line” on 4DN wiki. Consent for lines isolated prior to January 15, 2015 - DISCLAIMERPosting of public data from specimens collected before January 15, 2015 is the responsibility of and must be approved by each PIs institution. Additional questions about Consent should be addressed by individual PIs with their institutions. NIH page of FAQs for Genomic Data Sharing Policy: (EXAMPLE) Florida State University RESEARCH COMPLIANCE page Material Transfer Agreements (MTAs) - DISCLAIMERMTAs are the responsibility of each PI and their institution and are to be negotiated separately between each institution and the distributor. Additional questions about MTAs should be addressed by individual PIs with their institutions. The document below is a list of salient points from the MTAs between the OH (UCSD) and the three major providers, provided to 4DN PIs for your convenience as a courtesy and not to be considered a binding document. Readers Digest List of MTA Conditions Condensed by the OH ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?- please see this discussion group of who is engineering what so as not to duplicate effort or work together! How to Order Tier 1 and Tier 2 CellsIMPORTANT! The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells.
Before thawing the cells DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED “GOLD STANDARD” - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED “GOLD STANDARD”. MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!
Request a second vial: Request a letter of authorization from the OH (oh@4dnucleome.org) and send it by email to Tennielle Ludwig from WiCell (tludwig@wicell.org). After you have send the letter the order procedure is the same. 2. GM12878, reserved lot by Coriell: AVAILABLE Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase
Request a second vial: Request a letter of authorization from the OH (oh@4dnucleome.org) and send it by email to Eunice Horton (ehorton@coriell.org) Deborah Requesens (alternate contact) from Coriell (drequesens@coriell.org). After you have send the letter the order procedure is the same.
3. IMR90, reserved lot by ATCC: AVAILABLE Process for international customers: Step 1: 1. Consortium members will receive product availability email from ATCC with a fillable 4DN MTA Addendum https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing 2.Members will sign the MTA and send it to Stefan Brings (sbrings@atcc.org). Step 2: 1. Stefanwill send theATCC Order Form through email. 2. 4DN Member will complete the order form and send it to sales@atcc.org or Fax (703-365-2750) it for a quote (for shipping cost basically). 3. ATCC international order entry team will process the order. Request a second vial: Request a letter of consent from Dr. David Gilbert (gilbert@bio.fsu.edu) and send it by email to Stefan Brings from ATCC (sbrings@atcc.org) 4. HFF-hTert-clone 6 reserved lot from UMass. AVAILABLE The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge. The recipient PI will pay for the shipping of the cells using FedEx or UPS To request the cells, please contact Johan Gibcus (johan.gibcus@umassmed.edu) from UMass to set up MTA 5. F121-9 hybrid CASTx129 Female Mouse ESC: AVAILABLE Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions. NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: takahash@wi.mit.edu 6. F123 hybrid CASTx129 Male Mouse ESC: AVAILABLE Contact Bing Ren for vials or with questions. NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: takahash@wi.mit.edu 7. HAP1 Cells: AVAILABLE HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute. The PI is responsible of the shipping cost To order the cells and sign MTA contact Dr. Bas van Steensel by email: b.v.steensel@nki.nl 8. Human embryonic stem cells H9 (female), by WiCell. AVAILABLE Obtain from WiCell, use SOP listed on Cells Wiki 9. hTERT-RPE: AVAILABLE Obtain from ATCC, use SOP listed on Cells Wiki 10. K562: AVAILABLE Obtain from ATCC, use SOP listed on Cells Wiki 11. HEK293: AVAILABLE Obtain from ATCC, use SOP listed on Cells Wiki 12. WTC-11: AVAILABLE Obtain from Coriell, use SOP listed on Cells Wiki 13. U2OS: AVAILABLE Obtain from ATCC, use SOP listed on Cells Wiki 14. JM8.N4: AVAILABLE Obtain from KOMP, use SOP listed on Cells Wiki 15. G4 mESC: AVAILABLE Contact Carol Ware from the ISCRM-University of Washinton for G4 mESC distribution cware@u.washington.edu Execute and sign Material Transfer Agreement (MTA) with Mt. Sinai contact Michael Hanna by email: hanna@lunenfeld.ca The PI is responsible for the shipping cost Use SOP listed on Cells Wiki
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4dn/phase1/4dn_cell_repository.txt · Last modified: 2025/04/22 16:21 (external edit)