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4dn:phase1:4dn_cell_repository
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4dn:phase1:4dn_cell_repository [2020/07/01 08:10] oh |
4dn:phase1:4dn_cell_repository [2025/04/22 16:21] (current) |
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| <font 14px/inherit;;inherit;;inherit>**Content of your request letter**</font> | <font 14px/inherit;;inherit;;inherit>**Content of your request letter**</font> | ||
| - | In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells.~~CKG_TABLE_NBSP~~Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy. | + | In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells.~~CKG_TABLE_NBSP~~**Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.** |
| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
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| <font inherit/inherit;;#e74c3c;;inherit>**IMPORTANT!**</font>~~CKG_TABLE_NBSP~~The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. | <font inherit/inherit;;#e74c3c;;inherit>**IMPORTANT!**</font>~~CKG_TABLE_NBSP~~The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells. | ||
| - | <font 14px/inherit;;inherit;;inherit>**Before thawing the cells**</font>\\ \\ <font inherit/inherit;;#e74c3c;;inherit>**Read the finalized SOP for your cell line from**</font> ~~CKG_TABLE_NBSP~~[[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/working-groups-list/cell-lines-and-samples-wg|Cell lines WG wiki page]].~~CKG_TABLE_NBSP~~The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. | + | <font 14px/inherit;;inherit;;inherit>**Before thawing the cells**</font>\\ \\ <font inherit/inherit;;#e74c3c;;inherit>**Read the finalized SOP for your cell line from**</font> ~~CKG_TABLE_NBSP~~[[https://wiki.4dnucleome.org/4dn:working_groups:cell_lines_and_samples|Cell lines WG wiki page]].~~CKG_TABLE_NBSP~~The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel. |
| **<font inherit/inherit;;#e74c3c;;inherit>DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!</font>** | **<font inherit/inherit;;#e74c3c;;inherit>DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED "GOLD STANDARD" - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED "GOLD STANDARD". MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!</font>** | ||
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| ~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | ~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>Request a second vial:</font> | ||
| - | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]])~~CKG_TABLE_NBSP~~and send it by email to Deborah Requesens from Coriell ([[drequesens@coriell.org|]]).~~CKG_TABLE_NBSP~~After you have send the letter the order procedure is the same. | + | ~~CKG_TABLE_NBSP~~Request a letter of authorization from the OH ([[oh@4dnucleome.org|]])~~CKG_TABLE_NBSP~~and send it by email to Eunice Horton ([[ehorton@coriell.org|]]) Deborah Requesens (alternate contact) from Coriell ([[drequesens@coriell.org|]]).~~CKG_TABLE_NBSP~~After you have send the letter the order procedure is the same. |
| ~~CKG_TABLE_NBSP~~ | ~~CKG_TABLE_NBSP~~ | ||
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| **3.~~CKG_TABLE_NBSP~~IMR90**, reserved lot by ATCC:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> | **3.~~CKG_TABLE_NBSP~~IMR90**, reserved lot by ATCC:~~CKG_TABLE_NBSP~~<font inherit/inherit;;#e74c3c;;inherit>AVAILABLE</font> | ||
| - | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/document/d/1_mZaHK2e_5W3aREFt-NaA9G99yJgnyzt8cDzfxPwaug/edit?usp=sharing|ATCC guide to online ordering (IMR90)]] | + | * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/file/d/1hK0oOhnSCM4pFS9Na7zYbSWzQU17MX9r/view?usp=sharing|ATCC guide to online ordering (IMR90)]] |
| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1F47BRHsqP-SUOw2gAFXWN6basOSGTZpTQCtx3QGVTOA/edit#gid=0|IMR90 Cells PI List]] | ||
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| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/a/eng.ucsd.edu/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDo0OGNiMzU2NmE3Y2ZlYjQz|ATCC General MTA]] | ||
| - | * ~~CKG_TABLE_NBSP~~[[https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/goog_416570263|https://sites.google.com/a/4dnucleome.org/4d-nucleome-wiki/goog_416570263]]~~CKG_TABLE_NBSP~~[[https://drive.google.com/a/4dnucleome.org/file/d/0B1Uh14Y8H75dODNvdGFVZ2xsVjQ/view?usp=sharing|Material Transfer Agreement 4DN Addendum]]~~CKG_TABLE_NBSP~~ | + | * ~~CKG_TABLE_NBSP~~[[https://drive.google.com/a/4dnucleome.org/file/d/0B1Uh14Y8H75dODNvdGFVZ2xsVjQ/view?usp=sharing|Material Transfer Agreement 4DN Addendum]]~~CKG_TABLE_NBSP~~ |
| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/viewer?a=v&pid=sites&srcid=NGRudWNsZW9tZS5vcmd8NGQtbnVjbGVvbWUtd2lraXxneDoyODEzYjc0YzA4ODcxMjA|Certificate of Analysis for December 2016 batch of cells]] | ||
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| ~~CKG_TABLE_NBSP~~The PI is responsible of the shipping cost | ~~CKG_TABLE_NBSP~~The PI is responsible of the shipping cost | ||
| - | ~~CKG_TABLE_NBSP~~To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl ]] | + | ~~CKG_TABLE_NBSP~~To order the cells and sign MTA contact Dr. Bas van Steensel by email: [[b.v.steensel@nki.nl |b.v.steensel@nki.nl ]] |
| * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] | * ~~CKG_TABLE_NBSP~~[[https://docs.google.com/spreadsheets/d/1r3Jr0Lpuh3kZjnswEH-UYiqg2qpP3a7ipsuzSOlri6M/edit#gid=0|HAP1 Cells_PI List]] | ||
4dn/phase1/4dn_cell_repository.1593616257.txt.gz · Last modified: 2025/04/22 16:21 (external edit)