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4dn:phase1:protocols:damid
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4dn:phase1:protocols:damid [2019/02/15 10:35] rcalandrelli |
4dn:phase1:protocols:damid [2025/04/22 16:21] (current) |
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| - Lentiviral transduction of Dam-fusions and cell culture (as described in [[http://www.google.com/url?q=http://www.nature.com/nprot/journal/v2/n6/pdf/nprot.2007.148.pdf&sa=D&sntz=1&usg=AFQjCNEh9PqPNz5FfBLG66mEQ6hVZN-5iA|Nature Protocol]]). | - Lentiviral transduction of Dam-fusions and cell culture (as described in [[http://www.google.com/url?q=http://www.nature.com/nprot/journal/v2/n6/pdf/nprot.2007.148.pdf&sa=D&sntz=1&usg=AFQjCNEh9PqPNz5FfBLG66mEQ6hVZN-5iA|Nature Protocol]]). | ||
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| - DNA isolation, methylation-specific amplification and library preparation (see protocol below). | - DNA isolation, methylation-specific amplification and library preparation (see protocol below). | ||
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| - Bioinformatic pipeline to process reads into normalized data tracks (see protocol below). | - Bioinformatic pipeline to process reads into normalized data tracks (see protocol below). | ||
| The complete workflow of an example experiment (HFF) is added as well. | The complete workflow of an example experiment (HFF) is added as well. | ||
| - | {{filelist>:4dn:protocols:*.pdf}} | + | ------------------------------------------------------------------------------------------ |
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| + | {{medialist>4dn:protocols:damid:}} | ||
4dn/phase1/protocols/damid.1550255747.txt.gz · Last modified: 2025/04/22 16:21 (external edit)