This page describes the ordering process for the established cell lines of the 4DN Cell Repository. The established cell lines specifically refer to the cell lines that have been approved by the Cell Line WG, 4DN steering committee, NIH, and have been ordered by OH or created by 4DN appointed groups.

Please note that there are additional cell lines that are under discussion by the Cell Line WG.

Availability of Tier 1 cell lines from WiCell, Coriell, and ATCC

Eligibility information for PIs who are new to 4DN Cell Repository

Importantly, the 4DN Cell Line Working Group has established a protocol that specifies which 4DN PIs are eligible for ordering what cell lines. To become eligible, please send a letter via email to David Gilbert <gilbert@bio.fsu.edu>, co-chair of Cell Line Working group, and copy Kate Rivera-Noel <oh@4dnucleome.org>, a member of the 4DN Cell Line Working group.

Content of your request letter

In your letter please provide your name, institution, the 4DN cell lines that you would like to access and briefly state your proposed use of these cells. Please also include a statement that the data generated from 4DN cell lines will be released according to the 4DN consortium data submission and release policy.

Review of your letter

After receiving your request letter, the 4DN Cell Line Working group will discuss your eligibility, and once you are approved by the Cell Line working group, you will be notified and more importantly you will see your name in the “current PI list who have requested this cell line” on 4DN wiki.

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Posting of public data from specimens collected before January 15, 2015 is the responsibility of and must be approved by each PIs institution. Additional questions about Consent should be addressed by individual PIs with their institutions.

Sample Institutional Certification Form for Public sharing of data from specimens collected before January 15, 2015 that lack consent

NIH page of FAQs for Genomic Data Sharing Policy:

(EXAMPLE) Florida State University RESEARCH COMPLIANCE page

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MTAs are the responsibility of each PI and their institution and are to be negotiated separately between each institution and the distributor. Additional questions about MTAs should be addressed by individual PIs with their institutions. The document below is a list of salient points from the MTAs between the OH (UCSD) and the three major providers, provided to 4DN PIs for your convenience as a courtesy and not to be considered a binding document.

Readers Digest List of MTA Conditions Condensed by the OH

ARE YOU ENGINEERING ANY OF THE 4DN CELL LINES?- please see this discussion group of who is engineering what so as not to duplicate effort or work together!

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IMPORTANT! The H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC) cell lines have been ordered and paid through the 4DN Organization Hub (UC San Diego). WTC-11 is available for purchase at Coriell, the only source of these cells. Each Principal Investigator is responsible for completing and signing the MTAs and for payment of shipping and handling of the cells.

Before thawing the cells

Read the finalized SOP for your cell line from Cell lines WG wiki page. The finalized SOP will provide supplier name with the exact lot numbers for the media and matrigel.

DATASETS GENERATED USING THE APPROVED SOP PRECISELY AND PROVIDING THE REQUISITE METADATA WILL BE MARKED “GOLD STANDARD” - IF YOU DO NOT FOLLOW THESE PROTOCOLS STRICTLY THEN YOUR DATASETS WILL NOT BE DESIGNATED “GOLD STANDARD”. MAKE SURE TO CHECK THE CELLS WIKI PAGE EACH TIME YOU START AN EXPERIMENT FOR ANY UPDATES ON REAGENTS, ETC.!!

1. Human embryonic stem cells H1, by WiCell. AVAILABLE

WICell Ordering Process [WA01 (H1)] (supplementary slides) August 30, 2016. Note that each approved 4DN PI (see below) can order one vial for free, expect for paying the shipping cost.

WiCell International Ordering Process (H1)

Request a second vial:

Request a letter of authorization from the OH (oh@4dnucleome.org) and send it by email to Tennielle Ludwig from WiCell (tludwig@wicell.org). After you have send the letter the order procedure is the same.

2. GM12878, reserved lot by Coriell: AVAILABLE

Passcodes have been sent to all 4DN investigators that requested the GM12878 cells to authorize their purchase

Coriell Ordering Instructions (GM12878)

Protocol to Order GM12878 Cells from Coriell (slides)

International Orders: The same ordering process should be in place, whether the orders are domestic or international. The international investigators still need to provide their unique passcode in the remarks, and they still need to provide a valid shipping account or prepaid label to cover shipping costs. If you have any specific questions regarding shipping, please do not hesitate to reach out to our Customer Service staff, who would be more than glad to assist the PIs. Their contact information is customerservice@coriell.orgcustomerservice@coriell.org or call 1-856-757-4848.

Current PI List that has requested GM12878 Cells as March09, 2018

Request a second vial:

Request a letter of authorization from the OH (oh@4dnucleome.org) and send it by email to Eunice Horton (ehorton@coriell.org) Deborah Requesens (alternate contact) from Coriell (drequesens@coriell.org). After you have send the letter the order procedure is the same.

3. IMR90, reserved lot by ATCC: AVAILABLE

ATCC guide to online ordering (IMR90)

IMR90 Cells PI List

ATCC General MTA

Material Transfer Agreement 4DN Addendum

Certificate of Analysis for December 2016 batch of cells

Current PI List that has Requested IMR90 cells_March 24, 2017

Process for international customers:

Step 1: 1. Consortium members will receive product availability email from ATCC with a fillable 4DN MTA Addendum

https://drive.google.com/file/d/0B1Uh14Y8H75dd3BKSUYweUtKZFk/view?usp=sharing

2.Members will sign the MTA and send it to Stefan Brings (sbrings@atcc.org).

Step 2: 1. Stefan will send theATCC Order Form through email.

2. 4DN Member will complete the order form and send it to sales@atcc.org or Fax (703-365-2750) it for a quote (for shipping cost basically).

3. ATCC international order entry team will process the order.

Request a second vial:

Request a letter of consent from Dr. David Gilbert (gilbert@bio.fsu.edu) and send it by email to Stefan Brings from ATCC (sbrings@atcc.org)

4. HFF-hTert-clone 6 reserved lot from UMass. AVAILABLE

The 4DN HFF-hTert-clone 6 Cells will be provided and distributed by Dr. Job Dekker Laboratory free of Charge.

The recipient PI will pay for the shipping of the cells using FedEx or UPS

To request the cells, please contact Johan Gibcus (johan.gibcus@umassmed.edu) from UMass to set up MTA

Ordering Process

5. F121-9 hybrid CASTx129 Female Mouse ESC: AVAILABLE

Contact Dave Gilbert, Bas van Steensel or Shoji Takahashi for cells. Dave Gilbert if any questions.

Simple Letter Agreement (SLA) for CASTx129

NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: takahash@wi.mit.edu

6. F123 hybrid CASTx129 Male Mouse ESC: AVAILABLE

Contact Bing Ren for vials or with questions.

Simple Letter Agreement (SLA) for CASTx129

NOTE: ShojiTakahashi is in charge of the MTAs for the hybrid CASTx129 cells. For more info, Shoji's email: takahash@wi.mit.edu

7. HAP1 Cells: AVAILABLE

HAP1 cells are provided and distributed by the Dr. van Steensel Laboratory from the Netherlands Cancer Institute.

The PI is responsible of the shipping cost

To order the cells and sign MTA contact Dr. Bas van Steensel by email: b.v.steensel@nki.nl

HAP1 Cells_PI List

8. Human embryonic stem cells H9 (female), by WiCell. AVAILABLE

Obtain from WiCell, use SOP listed on Cells Wiki

9. hTERT-RPE: AVAILABLE

Obtain from ATCC, use SOP listed on Cells Wiki

10. K562: AVAILABLE

Obtain from ATCC, use SOP listed on Cells Wiki

11. HEK293: AVAILABLE

Obtain from ATCC, use SOP listed on Cells Wiki

12. WTC-11: AVAILABLE

Obtain from Coriell, use SOP listed on Cells Wiki

13. U2OS: AVAILABLE

Obtain from ATCC, use SOP listed on Cells Wiki

14. JM8.N4: AVAILABLE

Obtain from KOMP, use SOP listed on Cells Wiki

15. G4 mESC: AVAILABLE

Contact Carol Ware from the ISCRM-University of Washinton for G4 mESC distribution cware@u.washington.edu

Execute and sign Material Transfer Agreement (MTA) with Mt. Sinai contact Michael Hanna by email: hanna@lunenfeld.ca

The PI is responsible for the shipping cost

Use SOP listed on Cells Wiki