The Transformative Collaborative Project Award (TCPA) provides research support to enable eligible investigators to pursue novel research directions or develop new technologies and tools that will further help the 4DN Program to achieve its stated goals. Awardees will use the TCPA to build unique trans- or interdisciplinary teams that are focused on finding solutions to complex or challenging biological problems relevant to the 4DN mission and to propose research or tool-development projects that complement existing 4DN efforts.

	Elucidating the role of nascent RNA in enhancer-promoter communication and three-dimensional genome organization PI: Karen Adelman, Co-PI: Joanna Wysocka Harvard Medical School
	Establishing the 3D chromatin architectural organization of the zebrafish embryonic genome PI: Brad Cairns, Co-PI: Antonio Giraldez University of Utah
	Multi-Contact Conformation Capture: uncovering regulatory hubs and mutually exclusive topologies PI: Wouter de Laat, Co-PI: Jeroen de Ridder Hubrecht Institute
	Tethered nuclease strategies for in situ mapping of 3D nuclear organization PI: Steve Henikoff, Co-PIs: Kamran Ahmad, Jay Shendure, William Noble Fred Hutchinson Cancer Research Center
	Inference and Validation of Chromosomes 3D Structure via Statistical Shape Analysis of Elastic Curves Models PI: Nicola Neretti, Co-PIs: Anuj Srivastava, Chao-Ting Wu Brown University
	The Cell Nucleus under Stress PI: Karla Neugebauer, Co-PI: Joan Steitz Yale University
	Dynamic 3D folding of the mammalian genome: molecular determinants and impact on gene expression in vivo PI: Francois Spitz, Co-PIs: Leonid Mirny, Thomas Gregor Institute Pasteur, Paris, France
	Development of TrAC-loop, a novel technique to detect genome-wide chromatin interactions PI: Keji Zhao NHLBI
	Accessing chromatin interactions by high-resolution analyses of correlated regulatory element variation PI: Tomi Pastinen, CO-PIs: Elin Grundberg, Guillaume Borque McGill University
	Spatiotemporal dynamics of chromatin: the circadian and aging connection PI: Arturas Petronis, CO-PI: Ting Wang Centre for Addiction and Mental Health, Canada