The 4DN Consortium Scientific Webinar Series is designed to promote sharing ideas and establishment of new collaborations among the consortium members. It features scientific presentations by 4DN member group. These presentations are focused on new discoveries, and new approaches and are not meant to be high-level summaries of deliverables/objectives of each project as in annual consortium meetings. Each group is strongly encouraged to present unpublished research. The PI of each member group may designate either themselves or members of their group to give the presentation.

The format of this webinar series will consist of hour-long online video conferences. In the first round of the Webinar series, 34 presentations are planned. Each Webinar will feature two presentations, with each presentation allocated 20’ of talk time followed by 10’ of discussion. All member groups of 4DN consortium are expected to present once in this round, although some NOFIC centers may present twice given the size of their grants.

In addition to monthly Webinar series, special one-time mini-symposiums may also be added to cover specific topics, as needed, at the discretion of the 4DN Steering Committee.

The Webinar Series will be on the 4th Friday of each Month from 8 am - 9 am Pacific Time, 11 am - 12 pm Eastern Time. The Webinar will consist of two presentations of 30 minutes each (20 minutes presentation, 10 minutes discussion).

The Webinar organization committee has discussed and come up with a schedule for presentations after Feb 2017. See the schedule here: 2017 Webinar Series Presentation Assignments.

The Webinar organization committee has discussed and come up with a schedule for presentations after March 2018. See the schedule here: 2018-2020 Webinar Series Presentation Assignments.

The schedule is ranked by funded project and the corresponding PI's last name. While the corresponding PI is put in the presentation slot, it is up to the corresponding PI to decide who in their team is to give the talk. It is encouraged that trainees are given the chance to present. If the corresponding PI wants to switch with someone else on the list in a mutual agreement, then she/he has the responsibility to arrange it and let the Webinar organization committee know.

Scientific Webinar Committee:

Burak Alver

Andrew Belmont

Jennifer Phillips-Cremins

Bing Ren (chair)

April 24, 2020

Presenter: Kami Ahmad (Fred Hutchinson Cancer Research Center) - “CUT&RUN and CUT&Tag methods for profiling chromatin marks and chromatin structure.” (Link to presenter slides)

Presenter: Jiang Xu (University of Southern California) - “Cryo-Milling TCC-Based Mapping of Genome-Wide Chromatin Contacts” (Link to presenter slides)

Previous Scientific Webinar presentations can be downloaded from the Scientific Webinar presentation archive page linked here.

8:00-8:20 am Pacific Time , 20 minutes talk, then 10 minutes Q&A

Kami Ahmad

Principal Investigator

kahmad@fredhutch.org

Title: “CUT&RUN and CUT&Tag methods for profiling chromatin marks and chromatin structure.”

Kami Ahmad is a principal investigator at the Fred Hutchinson Cancer Research Center working with Steven Henikoff's group. We work on technology development for chromatin profiling, including CUT&RUN and CUT&Tag methods. I apply these methods to deciphering the roles of chromatin silencing mechanisms in development.

8:30-8:50 am Pacific Time , 20 minutes talk, then 10 minutes Q&A

Jiang Xu

Postdoc Researcher

foxjuly@gmail.com

Title: “Cryo-Milling TCC-Based Mapping of Genome-Wide Chromatin Contacts”

I am a PostDoc Researcher in Dr. Lin Chen’s Research Group in University of Southern California. I got my degree in Clinical Medicine in 2004 and Ph.D. in Neurology in 2009 in China. Before jumping in the 3D nuclear structure field, I mainly focused on recombinant protein expression, purification and X-ray structural determination. I started to develop the cryo-milling based 3C method 4 years ago and have been doing so ever since.

The 4th Friday of every month, from Friday, January 26, 2018, to no end date
8:00 am | Pacific Daylight Time (San Francisco, GMT-07:00) | 1 hr

Click on this link to connect to the meeting: https://4dnucleome.webex.com/4dnucleome/j.php?MTID=md022feb98e8a2583384c2f07114ac51c

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Meeting number: 807 023 120
Meeting password: pb9MpJc8
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Click on the blue link - Join WebEx Meeting(above, not here) and you will be taken to a new window and asked to write your name and email - then click join. You might need to download the WebEx add on if you never used WebEx before. You will be taken to another window where you should choose call using computer (left circle) then you should be connected.

During Presentation:

ATTENDEES, PLEASE:

• MUTE YOUR COMPUTER MICROPHONE WHEN JOINING THE WEBINAR
• LET THE MODERATOR KNOW THAT YOU HAVE A QUESTION VIA CHAT
• QUESTIONS WILL BE ADDRESSED AT THE END OF EACH PRESENTATION, DURING THE DISCUSSION PERIODS.

In case we experience technical difficulties during the Webinars:

1. A new WebEx meeting invitation will be sent out immediately to the 4dn-network@4dnucleome.org email list.

2. If the problem with WebEx persists, OH will notify participants via 4dn-network@4dnucleome.org email to call 1.866.740.1260 and enter access code 8221626.

3. Participants can find the Scientific Webinar slides presentations above under Scientific Webinar Series slides presentations.

When: May 22, 2020

Moderated by: Bing Ren

Member from Yijun Ruan's group (Presenter: Minji Kim)

Member from Jay Ashok Shendure (contact), William Noble's group (Presenter: Giancarlo Bonora)