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4dn:phase1:working_groups:omics_data_standards

Omics Data Standards

The mailing list for the working group has been changed. Please use omics@4dnucleome.org from now on as the new mailing list address.

Introduction

The Omics Data Standard Working Group will establish common standards, quality metrics, and data formats for the Omics experiments, datasets, and feature annotations to be produced and released by the 4DN consortium.

Mission / Objectives

The Omics Data Standard Working Group will establish common standards, quality metrics, and data formats for the Omics experiments, datasets, and feature annotations to be produced and released by the 4DN consortium.

Omics Standards Discussion Group

The Omics Standards Discussion Group (click link to follow) has been created to enable discussions on detailed experimental parameters (for example, ChIP-Seq antibodies) for all technologies involved within the network.

All 4DN members are encouraged to apply if their work involves any of the related technologies. If you wish to receive email updates for everything happening in this group, please use the button “Apply to join group” above, set your email preferences in “Email delivery preference” in the popped up panel, tell us who you are (name and affiliation) and, if you are not a 4DN PI, which 4DN PI you are associated with, then click Apply to join this group.

Meetings

Omics conference meetings are held twice a month via WebEx, on the 2nd and 4th Monday of each month at 11am PST/1pm CST/2pmEST. Each call lasts approximately 1 hour. WebEx conference calls information is below.

Please use the following link to incorporate edits and protocol changes ahead of time:

https://docs.google.com/document/d/1BBHkwrilTaL6N8dQ-QJE5KXKRrZaox3hD7XdEoVyII8/edit

WebEx info Call-Second Monday

The 2nd Monday of every month from Monday, March 14, 2016, to Monday December 11, 201

11:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 805 470 441

Meeting password: KME4Q7Ew

Join by phone

+1-415-655-0002 US Toll

Access code: 805 470 441

WebEx info Call-Fourth Monday

The 4th Monday of every month from Monday, May 23, 2016 , to no end date

11:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 807 058 449

Meeting password: pJhzCW2m

Join by phone

+1-415-655-0002 US Toll

Access code: 807 058 449

The Allele Specific Omics Data Standards Group will meet 2nd and 4th Wednesdays of the month at 10AM PT (1PM EST). The first call will be on Nov 22, 2017.

WebEx info Call-Second Wednesday

The 2nd Wednesday of every month from Wednesday, December 13, 2017, to no end date

10:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 804 302 182

Meeting password: PTPvVvF8

Join by phone

+1-415-655-0002 US Toll

Access code: 804 302 182

WebEx info Call-Fourth Wednesday

The 4th Wednesday of every month from Wednesayday, November 22, 2017, to no end date

10:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 806 649 089

Meeting password: 9rkJs4Mb

Join by phone

+1-415-655-0002 US Toll

Access code: 806 649 089

The Single Cell Standards Group will meet 1st and 3rd Wednesdays of the month at 9AM PT (12PM EST). The first call will be on Nov 15.

WebEx info Call-First Wednesday

The 1st Wednesday of every month from Wednesday, December 6, 2017, to no end date

9:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 801 959 044

Meeting password: UvGRxga4

Join by phone

+1-415-655-0002 US Toll

Access code: 801 959 044

WebEx info Call- Third Wednesday

The 3rd Wednesday of every month from Wednesday, November 15, 2017 , to no end date

9:00 am| Pacific Daylight Time (San Francisco, GMT-07:00| 1hr

Join WebEx meeting

Meeting number: 805 001 599

Meeting password: Ustw3jZi

Join by phone

+1-415-655-0002 US Toll

Access code: 805 001 599

WEBEX CONTACT INFORMATION:

Catherine Rivera-Noel oh@4dnucleome.org

Documents

Meeting Date Agenda Minutes

12-11-2015

  • Discussion of mission
2-8-2016Erez gave a presentation focused on the analysis pipeline of in situ HiC data. The software includes: mapping, normalization, reproducibility analysis, domain calls, loop calls, and visualization. The package is publicly available at GitHub.
2-22-2016
3-14-2016  
3-28-2016  
5-23-2016
  • Discussion of data file format specifics
6-13-2016
  • Discussion of experiment protocols
  • Discussion of standard data formats

Meeting minutes
Google docs links for experiment protocols and data formats, for suggestions and comments

6-27-2016
  • Determine experimental protocols
  • Discussion of meta-data
  • Discussion of data formats
7-11-2016 
7-25-2016
  • Discuss about file formats
8-8-2016
  • Discuss about file formats and benchmark methods / results
8-22-2016
9-12-2016
9-26-2016
  • OMICS year 1 report
  • Dataset requirements (coverage, etc.)
10-24-2016
  • Hi-C data standards (replicates, sequencing depth)
11-14-2016
  • Finalizing data standards for Hi-C
11-28-2016
  • ChIA-PET protocols and standards

Meeting minutes
Presentation

12-12-2016
  • Definition of loops

Meeting minutes
Presentation from Suhas Rao

1-9-2017
  • Discussion of ChIA-PET data standard and guidelines
2-13-2017
  • Discussion of ChIA-PET and PLAC-seq

Presentation from Rongxin Fang
Meeting minutes

2-27-2017
  • Discussion of future OMICS WG plans
3-13-2017
  • Discussion about Repli-seq
4-10-2017
  • Hi-C data submission and processing plans
4-24-2017
  • Alignments of Hi-C
5-8-2017
  • Hi-C Alignments and Normalization Procedures
5-22-2017
  • Repli-Seq protocol and standards. (Protocol, Standards)
    OMICS members on call has approved the E/L Repli-seq documents through Step 9 and they will be sent to 4DN SC for final approval. An updated Step 10 will be jointly presented by DCIC and Gilbert Lab and be approved later.
  • Hi-C Read Alignment
6-12-2017
  • Discussion of HG38 vs. HG19. Which version should be used by 4DN? Pros and cons
  • Concepts of chromatin loops, domains. Collection of opinions.
6-26-2017
  • Discussion of norm test (Burak/DCIC).
  • Finishing up discussion of hg19 vs hg38,
  • Compartment calling
7-10-2017
  • Discussion of norm test (Burak/DCIC).
7-24-2017
  • Discussion an updated ChIA-PET protocol and guideline (Yijun Ruan)

ChIA-PET updated protocol
ChIA-PET slides
Meeting minutes

8-14-2017
  • Continued discussion an updated ChIA-PET protocol and guideline (Yijun Ruan)
  • A proposed DCIC HI-C processing pipeline (Peter Park)
8-28-2017
  • Continue discussion with Chia-PET data analysis
  • Continue discussion DCIC proposal regarding Hi-C visualization and process pipeline
10-9-2017
  • Identify related techniques that are practiced by multiple groups, and form sub-groups for the drafting of experimental protocols and data standards of these methods.
  • Identify techniques that are being practiced by single labs, and schedule the discussion protocol/data standards.
10-23-2017
  • Discuss about the strategies for single cell Hi-C and allelic analysis data sets.
11-13-2017
  • DNase-HiC protocol (Vijay Ramani from Jay Shendure lab)
  • Discussion
  • Single cell combinatorial indexing Hi-C protocol (sciHi-C)
11-27-2017
  • Update on PLAC sub working group discussion (Miao Yu)
  • Update on single cell Hi-C (Burak Alver)
  • Update on allelic analysis of Omics data (Burak Alver or someone)
  • Feedbacks and discussion of DNase-HiC and sciHi-C
1-8-2018
  • Update on the sub-working group focusing on PLAC-seq standard (Miao Yu)
  • Update on the sub-working group focusing on single cell Hi-C discussion (Burak Alver)
  • Update on the sub-working group focusing on allelic analysis of HiC and other OMICS data (Burak Alver)
  • Vote to recommend the approval of the Dnase-HiC protocol (link here). This protocol was presented by Vijay Ramani from Jay Shendure lab on November 13.
1-22-2018
  • Update from sub-working group on PLAC-seq/HiChIP (Miao Yu)
  • Update from sub-working group on single cell Hi-C (Burak Alver)
  • Presentation of SPRITE (Sofia Quinodoz/Guttman Lab) followed by discussion.
2-12-2018
  • Updates on the sub-working groups.
  • Potential feedbacks on the SPRITE protocol.
  • Scheduling of additional OMICS protocols for future discussion.
2-26-2018
  • Updates on the sub working groups
3-12-2018
  • Presentation by Peter Fraser, single cell HI-C method.
  • Update on sub working groups:
  • PLAC-seq/HiChIP: (Miao Yu)
  • single cell Hi-C (Burak)
  • other sub working groups
4-9-2018
  • Discussion and approval of Single Cell Hi-C protocol (link), presented by Dr. Peter Fraser a month ago.
  • Update from sub-working group - PLAC-seq/HiChIP (Miao Yu). Different library prep will not affect data quality.
  • Update from sub-working group - single cell Hi-C (Burak Alver).
  • Update from sub-working group - allele specific Hi-C (Burak Alver).
  • Other businesses for future OMICS sessions
5-14-2018
  • Overview of TSA-Seq method (Andrew Belmont’s research group).
6-11-2018
  • Presentation on DamID protocol and data standard by Tom van Schaik (Bas van Steensel lab).
6-25-2018
  • Discussion on CUT&RUN by Steven Henikoff
7-23-2018
  • Discuss and vote on the DamID protocol (see link here). This was presented on June 11th.
  • Discussion and vote on the Cut&Run protocol (see link here). This was presented on June 25th.
  • Allele-specific alignment of HiC reads. (Burak Alver).
8-13-2018
  • PLAC-seq data analysis by Ivan Juric and Miao Yu.
9-10-2018
  • Update on PLAC-seq/HiChIP standard discussion (Miao Yu)
  • Future OMICS discussion and presentation sign ups.
11-19-2018
  • Presentation on optimization and comparison of PLAC-seq and HiChIP protocols (Ren lab/Miao Yu).
  • Discussion.

Meeting minutes
Presentation by Miao Yu. “Optimization and Comparison of PLAC-seq and HiChIP protocols”

1-28-2019
  • PLAC-seq/HiChIP discussion update (Miao Yu)
  • Single cell Hi-C sub-working group (Burak / Bill)
  • Plan out the OMICS working group for 2019
2-25-2019
  • Schedule for future presentations
  • Need to look at the SC minutes to see which protocols are in production, and approved. (DNase-HiC, in situ HiC, DamID, repli-seq, ChIA-PET)
  • Any additional omic standards to be discussed?
3-25-2019
  • Update on PLAC-seq / HiChIP protocol

Meeting minutes

Presentation by Miao Yu

2020 Meetings

3-23-2020
  • Review protocols recommended for 4DN SC
4-27-2020
  • Review last month’s minutes and action plan.
  • Presentation on comparison of ChIA-PET/HiCHIP/PLAC-seq (Dr. Xiaotao Wang from Dr. Feng Yue’s group)
6-22-2020 
  • Meeting minutes 

Mailing list

The mailing list to use for communication in this Working Group is omics@4dnucleome.org. Emails sent there will go to everyone on the list. Replying to an email from the list will also send it to everyone in the list.

Calendar


4dn/phase1/working_groups/omics_data_standards.txt · Last modified: 2025/04/22 16:21 (external edit)